Modulating alternative splicing by cotranscriptional

被引：16

作者：

Gromak, Natalia ^{[1
]}

Talotti, Garriele ^{[2
]}

Proudfoot, Nicholas J. ^{[1
]}

Pagani, Franco ^{[2
]}

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

[2] Int Ctr Genet Engn & Biotechnol, I-34012 Trieste, Italy

来源：

RNA | 2008年 / 14卷 / 02期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

alternative splicing regulation; RNA polymerase II; alpha-tropomyosin gene; fibronectin gene; ribozyme;

D O I：

10.1261/rna.615508

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cotranscriptional cleavage mediated by a hammerhead ribozyme can affect alternative splicing if interposed between an exon and its intronic regulatory elements. This has been demonstrated using two different alternative splicing systems based on alpha-tropomyosin and fibronectin genes. We suggest that there is a requirement for intronic regulatory elements to be covalently attached to exons that are in turn tethered to the elongating polymerase. In the case of the alternatively spliced EDA exon of the fibronectin gene, we demonstrate that the newly identified intronic downstream regulatory element is associated with the splicing regulatory protein SRp20. Our results suggest that targeted hammerhead ribozyme cleavage within introns can be used as a tool to define splicing regulatory elements.

引用

页码：359 / 366

页数：8

共 30 条

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