Molecular cytogenetic analysis of genome structure in Lupinus angustifolius and Lupinus cosentinii

被引：51

作者：

Hajdera, I ^{[1
]}

Siwinska, D ^{[1
]}

Hasterok, R ^{[1
]}

Maluszynska, J ^{[1
]}

机构：

[1] Univ Silesia, Dept Plant Anat & Cytol, PL-40032 Katowice, Poland

来源：

THEORETICAL AND APPLIED GENETICS | 2003年 / 107卷 / 06期

关键词：

FISH; flow cytometry; Lupinus; rDNA; telomere;

D O I：

10.1007/s00122-003-1303-3

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Molecular cytogenetic analysis of Lupinus angustifolius and Lupinus cosentinii was performed using flow cytometry, fluorescence in situ hybridisation (FISH) and differential chromosome staining. Genome size was determined as 2.07 pg for L. angustifolius and 1.54 pg for L. cosentinii. Analysis of nuclear DNA amount in cells during plant development has shown endopolyploidisation in different organs. The highest level of endopolyploidy was in cotyledons and reached 32C in L. angustifolius and 64C in L. cosentinii. Both of the investigated Lupinus species belong to the polysomatic type of plants. Double FISH with rDNA probes provided chromosomal landmarks for 10 out of 40 chromosomes for L. angustifolius and 8 out of 32 chromosomes for L. cosentinii. In L. angustifolius, the number and localisation of 25S rDNA hybridisation signals precisely corresponded to the chromomycin A3 (CMA(+)) bands, while in L. cosentinii both 25S and 5S rDNA loci overlapped with CMA(+) bands. Silver staining revealed that only 45S rRNA genes located in secondary constriction regions were transcriptionally active. FISH with Arabidopsis-type telomeric arrays revealed the presence of signals at termini of all chromosomes. Despite the application of different DNA probes for FISH and different chromosome staining, a relatively small proportion of chromosomes in the Lupinus karyotypes can be distinguished. Identification of all chromosomes requires the use of more chromosome-specific markers.

引用

页码：988 / 996

页数：9

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