Antigen retrieval, signal amplification and intensification in immunohistochemistry

被引：128

作者：

Werner, M ^{[1
]}

vonWasielewski, R ^{[1
]}

Komminoth, P ^{[1
]}

机构：

[1] UNIV ZURICH, DEPT PATHOL, DIV CELL & MOL PATHOL, ZURICH, SWITZERLAND

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 1996年 / 105卷 / 04期

关键词：

D O I：

10.1007/BF01463928

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In this overview we emphasize new methods of improving immunohistochemical results in formaldehyde-fixed tissue samples. The benefit of heat-induced antigen retrieval in demasking of concealed epitopes is demonstrated. We provide guidance on the influence of heat-induced antigen retrieval in commonly applied monoclonal and polyconal antibodies. Moreover, we show the promising methods of signal amplification using biotinylated tyramine and signal intensification of diaminobenzidine reaction products by metallic ions.

引用

页码：253 / 260

页数：8

共 55 条

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