Gordonan, an acidic polysaccharide with cell aggregation-inducing activity in insect BM-N4 cells, produced by Gordonia sp.

被引：19

作者：

Kondo, T

Yamamoto, D

Yokota, A

Suzuki, A

Nagasawa, H

Sakuda, S ^{[1
]}

机构：

[1] Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan

[2] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 11期

关键词：

Gordonia; polysaccharide; cell aggregation; insect cell line;

D O I：

10.1271/bbb.64.2388

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An acidic polysaccharide, termed gordonan, was isolated from the culture medium of Gordonia sp. as an inducer of cell aggregation in an insect cell line, BM-N4. Gordonan had an average molecular weight of 5X10(6) and its structure was identified as -->3)-4-O-(1-carboxyethyl)-beta-D-Manp-(1-->4)-beta-D-GlcAp-(1-->4)-beta-D-Glcp-(1--> mainly by acid hydrolysis experiments and NMR analysis. It induces cell aggregation at the concentration of 4 mug/ml. A partially hydrolyzed polysaccharide derived from gordonan with a molecular weight of 5X10(5) showed weak activity, while any fragment molecules with lower molecular weights prepared from gordonan showed no activity.

引用

页码：2388 / 2394

页数：7

共 20 条

[11] The major 20-kDa polysaccharide of Staphylococcus epidermidis extracellular slime and its antibodies as powerful agents for detecting antibodies in blood serum and differentiating among slime-positive and -negative S-epidermidis and other staphylococci species [J].