Metabolic engineering for pentose utilization in Saccharomyces cerevisiae

被引:192
作者
Hahn-Haegerdal, Bdrbel [1 ]
Karhumaa, Kaisa [1 ]
Jeppsson, Marie [1 ]
Gorwa-Grauslund, Marie F. [1 ]
机构
[1] Lund Univ, S-22100 Lund, Sweden
来源
BIOFUELS | 2007年 / 108卷
关键词
arabinose; ethanol; fermentation; lignocellulose; xylose; yeast;
D O I
10.1007/10_2007_062
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The introduction of pentose utilization pathways in baker's yeast Saccharomyces cerevisiae is summarized together with metabolic engineering strategies to improve ethanolic pentose fermentation. Bacterial and fungal xylose and arabinose pathways have been expressed in S. cerevisiae but do not generally convey significant ethanolic fermentation traits to this yeast. A large number of rational metabolic engineering strategies directed among others toward sugar transport, initial pentose conversion, the pentose phosphate pathway, and the cellular redox metabolism have been exploited. The directed metabolic engineering approach has often been combined with random approaches including adaptation, mutagenesis, and hybridization. The knowledge gained about pentose fermentation in S. cerevisiae is primarily limited to genetically and physiologically well-characterized laboratory strains. The translation of this knowledge to strains performing in an industrial context is discussed.
引用
收藏
页码:147 / 177
页数:31
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