The HSP70 heat shock response in the Antarctic fish Harpagifer antarcticus

被引：61

作者：

Clark, Melody S. ^{[1
]}

Fraser, Keiron P. P. ^{[1
]}

Burns, Gavin ^{[1
]}

Peck, Lloyd S. ^{[1
]}

机构：

[1] British Antarctic Survey, NERC, Cambridge CB3 0ET, England

来源：

POLAR BIOLOGY | 2008年 / 31卷 / 02期

基金：

英国自然环境研究理事会;

关键词：

Antarctic; climate change; stenothermal; heat shock protein; marine;

D O I：

10.1007/s00300-007-0344-5

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Members of the HSP70 gene family comprising the constitutive (HSC70) and inducible (HSP70) genes, plus GRP78 (Glucose-regulated protein 78 kDa) were surveyed for expression levels via Q-PCR after both an acute 2-h heat shock experiment and a time course assay in the Antarctic plunderfish Harpagifer antarcticus. In general, down regulation of all genes was observed during the course of the heat shock experiments. This thermally induced down regulation was particularly acute for the GRP78 gene, which at one time point was more than 100-fold down regulated. These results demonstrate the loss of the heat shock response in H. antarcticus, a basal member of the Notothenioidei. This finding is discussed with reference to the survival of Notothenioids during observed ocean warming and also the reorganisation of cellular protein mechanisms of species living in extreme environments.

引用

页码：171 / 180

页数：10

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