Two-year stability of NT-proBNP in frozen samples using the Roche Elecsys system

被引：37

作者：

Cauliez, B. ^{[1
]}

Guignery, J. ^{[1
]}

Marinier, S. ^{[1
]}

Mariau, I. ^{[1
]}

Lavoinne, A. ^{[1
]}

机构：

[1] CHU Rouen, Hop Charles Nicolle, Lab Biochim Med, F-76031 Rouen, France

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 2008年 / 45卷

关键词：

D O I：

10.1258/acb.2007.007187

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: The in vitro stability of N-terminal (NT)-proBNP (brain natriuretic peptide) at room temperature and at 4 degrees C is excellent and has been well studied. However, less is known concerning its stability after a long-term frozen storage. This notion could be of major interest in the context of clinical evaluations. Methods: NT-proBNP was measured on 97 heparinized samples before and after a two-year frozen storage (- 20 degrees C) using the Roche Elecsys system. Results: There is a slight but significant decrease of NT-proBNP concentration after frozen storage. However, this decrease is <10% for more than 90% of the samples and the maximum decrease is 16%. Moreover, values on frozen samples are well correlated with values on fresh samples (r = 0.99) using the following equation: NT-proBNP(stored) (sample) = 0.93 NT-proBNP(fresh sample) + 81. A similar equation was found in lower concentrations (NT-proBNP <= 2000 ng/L). Conclusions: These data show that NT-proBNP can be stored for at least two years at -20 degrees C before measurement without a substantial loss of immunoreactivity.

引用

页码：318 / 319

页数：2

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