A comparison of gel-based, nylon filter and microarray techniques to detect differential RNA expression in plants

被引：84

作者：

Baldwin, D ^{[1
]}

Crane, V ^{[1
]}

Rice, D ^{[1
]}

机构：

[1] Pioneer HiBred Int Inc, Trait & Technol Dev, Dis Resistance Grp, Johnston, IA 50131 USA

来源：

CURRENT OPINION IN PLANT BIOLOGY | 1999年 / 2卷 / 02期

关键词：

D O I：

10.1016/S1369-5266(99)80020-X

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An initial application of plant genomics has been to monitor gene expression on a scale much larger than previously possible. Although multiplexed assays of RNA abundance have developed more quickly than those for protein and metabolite levels, some combination of these approaches will soon be providing our best views yet into plant molecular biology. Three techniques that have made contributions to the RNA transcript portion of this combination are reviewed. Currently, each can produce a profile of expression levels for a large but incomplete set of plant genes, at reproducibly high levels of accuracy and over a range of labor and financial expenses.

引用

页码：96 / 103

页数：8

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