Combining x-ray crystallography and electron microscopy

被引：93

作者：

Rossmann, MG ^{[1
]}

Morais, MC ^{[1
]}

Leiman, PG ^{[1
]}

Zhang, W ^{[1
]}

机构：

[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

来源：

STRUCTURE | 2005年 / 13卷 / 03期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1016/j.str.2005.01.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of cryo-electron microscopy to study large biological assemblies at low resolution with crystallography to determine near atomic structures of assembly fragments is quickly expanding the horizon of structural biology. This technique can be used to advantage in the study of large structures that cannot be crystallized, to follow dynamic processes, and to "purify" samples by visual selection of particles. Factors affecting the quality of cryo-electron microscopy maps and limits of accuracy in fitting known structural fragments are discussed.

引用

页码：355 / 362

页数：8

共 35 条

[1] Adding the third dimension to virus life cycles: Three-dimensional reconstruction of icosahedral viruses from cryo-electron micrographs [J].

Baker, TS ;

Olson, NH ;

Fuller, SD .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1999, 63 (04) :862-+

[2] Macromolecular electron microscopy in the era of structural genomics [J].

Baumeister, W ;

Steven, AC .

TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (12) :624-631

[3] CRYO-ELECTRON MICROSCOPY OF VITRIFIED SPECIMENS [J].

DUBOCHET, J ;

ADRIAN, M ;

CHANG, JJ ;

HOMO, JC ;

LEPAULT, J ;

MCDOWALL, AW ;

SCHULTZ, P .

QUARTERLY REVIEWS OF BIOPHYSICS, 1988, 21 (02) :129-228

[4]

Eiserling Frederick A., 1994, P209

[5] Molecular architecture of the prolate head of bacteriophage T4 [J].

Fokine, A ;

Chipman, PR ;

Leiman, PG ;

Mesyanzhinov, VV ;

Rao, VB ;

Rossmann, MG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (16) :6003-6008

[6] Historical background: why is it important to improve automated particle selection methods? [J].

Glaeser, RM .

JOURNAL OF STRUCTURAL BIOLOGY, 2004, 145 (1-2) :15-18

[7] Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments:: implications of crowding [J].

Grünewald, K ;

Medalia, O ;

Gross, A ;

Steven, AC ;

Baumeister, W .

BIOPHYSICAL CHEMISTRY, 2003, 100 (1-3) :577-591

[8] Inter-RNA interaction of phage φ29 pRNA to form a hexameric complex for viral DNA transportation [J].

Guo, PX ;

Zhang, CL ;

Chen, CP ;

Garver, K ;

Trottier, M .

MOLECULAR CELL, 1998, 2 (01) :149-155

[9] Realizing the potential of electron cryo-microscopy [J].

Henderson, R .

QUARTERLY REVIEWS OF BIOPHYSICS, 2004, 37 (01) :3-13

[10] Bridging the information gap: Computational tools for intermediate resolution structure interpretation [J].

Jiang, W ;

Baker, ML ;

Ludtke, SJ ;

Chiu, W .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 308 (05) :1033-1044

← 1 2 3 4 →