Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli

被引:56
作者
Das, Gautam [1 ]
Thotala, Dinesh Kumar [1 ]
Kapoor, Suman [1 ]
Karunanithi, Sheelarani [1 ]
Thakur, Suman S. [1 ]
Singh, N. Sadananda [1 ]
Varshney, Umesh [1 ]
机构
[1] Indian Inst Sci, Dept Microbiol & Cell Biol, CNR Rao Circle, Bangalore 560012, Karnataka, India
关键词
S-adenosyl-L-methionine; 5,10 methylene tetrahydrofolate dehydrogenase; methyltransferase; modified nucleoside; folD;
D O I
10.1038/emboj.2008.20
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Translation initiation from the ribosomal P-site is the specialty of the initiator tRNAs ( tRNA fMet). Presence of the three consecutive G-C base pairs ( G29-C41, G30-C40 and G31-C39) in their anticodon stems, a highly conserved feature of the initiator tRNAs across the three kingdoms of life, has been implicated in their preferential binding to the P-site. How this feature is exploited by ribosomes has remained unclear. Using a genetic screen, we have isolated an Escherichia coli strain, carrying a G122D mutation in folD, which allows initiation with the tRNA(fMet) containing mutations in one, two or all the three G-C base pairs. The strain shows a severe deficiency of methionine and S-adenosylmethionine, and lacks nucleoside methylations in rRNA. Targeted mutations in the methyltransferase genes have revealed a connection between the rRNA modifications and the fundamental process of the initiator tRNA selection by the ribosome.
引用
收藏
页码:840 / 851
页数:12
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