Kinetics of desorption of proteins from the surface of protein-coated alumina by various desorbing reagents

The phenomena of desorption of adsorbed proteins have been studied in detail, The extents and rates of desorption of lysozyme from the surface of a protein-coated alumina by different desorbing reagents have been studied, The extent and rate of desorption of lysozyme preadsorbed at pH 7.0 are found to be maximum in the presence of 2 M NaCl solution and in the presence of a buffer of pH 5.0, The rate of desorption of three proteins, BSA, gelatin, and beta-lactoglobulin, preadsorbed at pH 5.0 on the surface of alumina by a buffer of pH 7.0, were also studied, As in the case of adsorption, the kinetic data fit into a first-order equation with two rate constants K-d(1) and K-d(2). The effect of temperature on the rate of desorption of BSA and lysozyme has been studied. The various activation energy parameters for the desorption of proteins have been calculated and a mechanism of protein desorption has been proposed. (C) 1996 Academic Press, Inc.