Identification of Rhodococcus equi using the polymerase chain reaction

被引：34

作者：

Bell, KS

Philp, JC

Christofi, N

Aw, DWJ

机构：

[1] Department of Biological Sciences, Napier University, Edinburgh

[2] Department of Biological Sciences, Napier University, Edinburgh EH10 5DT

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1996年 / 23卷 / 02期

关键词：

D O I：

10.1111/j.1472-765X.1996.tb00033.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two regions in the gene coding for 16S rRNA in Rhodococcus equi were selected as species-specific primer sequences for the polymerase chain reaction (PCR). PCR using these primers was tested against 10 strains of R. equi (including the type strain) and gave positive results for all but was negative for all other tested species of Rhodococcus; representatives of the most closely related genera and a number of other bacterial species. This method could therefore be used to identify this species which can infect the lungs or other organs of horses, pigs, humans and other animals.

引用

页码：72 / 74

页数：3

共 13 条

[1] AMPLIFICATION OF NUCLEIC-ACIDS BY POLYMERASE CHAIN-REACTION (PCR) AND OTHER METHODS AND THEIR APPLICATIONS [J].