Arabidopsis Transcription Factor ELONGATED HYPOCOTYL5 Plays a Role in the Feedback Regulation of Phytochrome A Signaling

被引:159
作者
Li, Jigang [1 ,2 ]
Li, Gang [2 ]
Gao, Shumin [2 ,3 ]
Martinez, Cristina [2 ]
He, Guangming [1 ,2 ]
Zhou, Zhenzhen [2 ]
Huang, Xi [2 ]
Lee, Jae-Hoon [2 ]
Zhang, Huiyong [2 ]
Shen, Yunping [1 ,2 ]
Wang, Haiyang [2 ]
Deng, Xing Wang [1 ,2 ]
机构
[1] Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, Beijing 100871, Peoples R China
[2] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[3] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
PLANT PHOTORECEPTORS PHYTOCHROME; LIGHT-INDUCED PHOSPHORYLATION; LOOP-HELIX PROTEIN; GENE-EXPRESSION; NUCLEAR-LOCALIZATION; FACTOR HY5; INTERACTING FACTOR-3; NEGATIVE REGULATOR; A PHOTORECEPTOR; BZIP PROTEIN;
D O I
10.1105/tpc.110.075788
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phytochrome A (phyA) is the primary photoreceptor responsible for perceiving and mediating various responses to far-red light in Arabidopsis thaliana. FAR-RED ELONGATED HYPOCOTYL1 (FHY1) and its homolog FHY1-LIKE (FHL) are two small plant-specific proteins essential for light-regulated phyA nuclear accumulation and subsequent phyA signaling processes. FHY3 and its homolog FAR-RED IMPAIRED RESPONSE1 (FAR1) are two transposase-derived transcription factors that directly activate FHY1/FHL transcription and thus mediate subsequent phyA nuclear accumulation and responses. Here, we report that ELONGATED HYPOCOTYL5 (HY5), a well-characterized bZIP transcription factor involved in promoting photomorphogenesis, directly binds ACGT-containing elements a few base pairs away from the FHY3/FAR1 binding sites in the FHY1/FHL promoters. We demonstrate that HY5 physically interacts with FHY3/FAR1 through their respective DNA binding domains and negatively regulates FHY3/FAR1-activated FHY1/FHL expression under far-red light. Together, our data show that HY5 plays a role in negative feedback regulation of phyA signaling by attenuating FHY3/FAR1-activated FHY1/FHL expression, providing a mechanism for fine-tuning phyA signaling homeostasis.
引用
收藏
页码:3634 / 3649
页数:16
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