TT virus mRNAs detected in the bone marrow cells from an infected individual

被引：65

作者：

Okamoto, H ^{[1
]}

Nishizawa, T

Tawara, A

Takahashi, M

Kishimoto, J

Sai, T

Sugai, Y

机构：

[1] Jichi Med Sch, Div Immunol, Minami Kawachi, Tochigi 3290498, Japan

[2] Jichi Med Sch, Div Mol Virol, Minami Kawachi, Tochigi 3290498, Japan

[3] Yamanashi Med Univ, Dept Internal Med 1, Kofu, Yamanashi 4093898, Japan

[4] Inst Immunol, Tokyo 1120004, Japan

[5] Iwaki Kyoritsu Gen Hosp, Dept Internal Med, Iwaki, Fukushima 9738555, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 279卷 / 02期

关键词：

TT virus; mRNA; replication; transcription; splicing; bone marrow cells;

D O I：

10.1006/bbrc.2000.4012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although replicative forms of TT virus (TTV) DNA have been found in the liver and bone marrow cells, mRNAs of TTV have not yet been detected in these tissues. The entire nucleotide sequence of a TTV clone [TYM9 (3759 bases)] isolated front a patient with high TTV viremia (10(6) copies/ml) was determined, and the poly(A)(+) RNAs from bone marrow cells were subjected to reverse transcription-polymerase chain reaction with primers specific for the TYM9 sequence. Sequence analysis of the amplified products revealed the presence of three distinct species of spliced TTV mRNAs [2.9, 1.2, and 1.0 kilobases (kb)] with common 5' and 3' termini as well as splicing to bind nucleotide (nt) 181 to nt 283. The shorter mRNAs of 1.2 kb and 1.0 kb possessed another splicing to join nt 681 with nt 2341 or nt 2579. The transcription profile of TTV found in an infected human corroborates that observed in vitro. (C) 2000 Academic Press.

引用

页码：700 / 707

页数：8

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