Potent Lentiviral Restriction by a Synthetic Feline TRIM5 Cyclophilin A Fusion

被引：15

作者：

Dietrich, Isabelle ^{[1
]}

Macintyre, Angela ^{[1
]}

McMonagle, Elizabeth ^{[1
]}

Price, Amanda J. ^{[2
]}

James, Leo C. ^{[2
]}

McEwan, William A. ^{[2
]}

Hosie, Margaret J. ^{[1
]}

Willett, Brian J. ^{[1
]}

机构：

[1] Univ Glasgow, Retrovirus Res Lab, Inst Comparat Med, Fac Vet Med, Glasgow G61 1QH, Lanark, Scotland

[2] MRC Lab Mol Biol, Div Prot & Nucle Acid Chem, Cambridge CB1 0QH, England

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 17期

基金：

英国惠康基金;

关键词：

IMMUNODEFICIENCY-VIRUS TYPE-1; TRIM5-ALPHA RESTRICTION; HIV-1; INFECTION; REVERSE TRANSCRIPTION; RHESUS TRIM5-ALPHA; HUMAN-CELLS; T-CELLS; REPLICATION; CAPSIDS; RECOGNITION;

D O I：

10.1128/JVI.00858-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A synthetic feline TRIM5-cyclophilin A fusion protein (feTRIMCyp) was generated and transduced into feline cells. feTRIMCyp was highly efficient at preventing infection with human (HIV) and feline (FIV) immunodeficiency virus pseudotypes, and feTRIMCyp-expressing cells resisted productive infection with either FIV-Fca or FIV-Pco. The restriction of FIV infection by feTRIMCyp was reversed by the cyclosporine (Cs) derivatives NIM811 and Debio-025 but less so by Cs itself. FeTRIMCyp and FIV infections of the cat offer a unique opportunity to evaluate TRIMCyp-based approaches to genetic therapy for HIV infection and the treatment of AIDS.

引用

页码：8980 / 8985

页数：6

共 40 条

[1] Stable reduction of CCR5 by RNAi through hematopoietic stem cell transplant in non-human primates [J].

An, Dong Sung ;

Donahue, Robert E. ;

Karnata, Masakazu ;

Poon, Betty ;

Metzger, Mark ;

Mao, Si-Hua ;

Bonifacino, Aylin ;

Krouse, Allen E. ;

Darlix, Jean-Luc ;

Baltimore, David ;

Qin, F. Xiao-Feng ;

Chen, Irvin S. Y. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (32) :13110-13115

[2] Catalysis of cis/trans isomerization in native HIV-1 capsid by human cyclophilin A [J].

Bosco, DA ;

Eisenmesser, EZ ;

Pochapsky, S ;

Sundquist, WI ;

Kern, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5247-5252

[3] Cyclophilin A regulates HIV-1 infectivity, as demonstrated by gene targeting in human T cells [J].

Braaten, D ;

Luban, J .

EMBO JOURNAL, 2001, 20 (06) :1300-1309

[4] Cyclophilin A is required for an early step in the life cycle of human immunodeficiency virus type 1 before the initiation of reverse transcription [J].

Braaten, D ;

Franke, EK ;

Luban, J .

JOURNAL OF VIROLOGY, 1996, 70 (06) :3551-3560

[5] Cyclophilin A is required for the replication of group M human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus SIV(CPZ)GAB but not group O HIV-1 or other primate immunodeficiency viruses [J].

Braaten, D ;

Franke, EK ;

Luban, J .

JOURNAL OF VIROLOGY, 1996, 70 (07) :4220-4227

[6] TRIMCyp expression in old world primates Macaca nemestrina and Macaca fascicularis [J].

Brennan, Greg ;

Kozyrev, Yury ;

Hu, Shiu-Lok .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (09) :3569-3574

[7] Visualization of a proteasome-independent intermediate during restriction of HIV-1 by rhesus TRIM5α [J].

Campbell, Edward M. ;

Perez, Omar ;

Anderson, Jenny L. ;

Hope, Thomas J. .

JOURNAL OF CELL BIOLOGY, 2008, 180 (03) :549-561

[8] Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1 [J].

Diaz-Griffero, Felipe ;

Vandegraaff, Nick ;

Li, Yuan ;

McGee-Estrada, Kathleen ;

Stremlau, Matthew ;

Welikala, Sohanya ;

Si, Zhihai ;

Engelman, Alan ;

Sodroski, Joseph .

VIROLOGY, 2006, 351 (02) :404-419

[9] Lessons from the cat: Feline immunodeficiency virus as a tool to develop intervention strategies against human immunodeficiency virus type 1 [J].

Elder, JH ;

Dean, GA ;

Hoover, EA ;

Hoxie, JA ;

Malim, MH ;

Mathes, L ;

Neil, JC ;

North, TW ;

Sparger, E ;

Tompkins, MB ;

Tompkins, WAF ;

Yamamoto, J ;

Yuhki, N ;

Pedersen, NC ;

Miller, RH .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 1998, 14 (09) :797-801

[10] SPECIFIC INCORPORATION OF CYCLOPHILIN-A INTO HIV-1 VIRIONS [J].

FRANKE, EK ;

YUAN, HEH ;

LUBAN, J .

NATURE, 1994, 372 (6504) :359-362

← 1 2 3 4 →