Potent Lentiviral Restriction by a Synthetic Feline TRIM5 Cyclophilin A Fusion

被引：15

作者：

Dietrich, Isabelle ^{[1
]}

Macintyre, Angela ^{[1
]}

McMonagle, Elizabeth ^{[1
]}

Price, Amanda J. ^{[2
]}

James, Leo C. ^{[2
]}

McEwan, William A. ^{[2
]}

Hosie, Margaret J. ^{[1
]}

Willett, Brian J. ^{[1
]}

机构：

[1] Univ Glasgow, Retrovirus Res Lab, Inst Comparat Med, Fac Vet Med, Glasgow G61 1QH, Lanark, Scotland

[2] MRC Lab Mol Biol, Div Prot & Nucle Acid Chem, Cambridge CB1 0QH, England

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 17期

基金：

英国惠康基金;

关键词：

IMMUNODEFICIENCY-VIRUS TYPE-1; TRIM5-ALPHA RESTRICTION; HIV-1; INFECTION; REVERSE TRANSCRIPTION; RHESUS TRIM5-ALPHA; HUMAN-CELLS; T-CELLS; REPLICATION; CAPSIDS; RECOGNITION;

D O I：

10.1128/JVI.00858-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A synthetic feline TRIM5-cyclophilin A fusion protein (feTRIMCyp) was generated and transduced into feline cells. feTRIMCyp was highly efficient at preventing infection with human (HIV) and feline (FIV) immunodeficiency virus pseudotypes, and feTRIMCyp-expressing cells resisted productive infection with either FIV-Fca or FIV-Pco. The restriction of FIV infection by feTRIMCyp was reversed by the cyclosporine (Cs) derivatives NIM811 and Debio-025 but less so by Cs itself. FeTRIMCyp and FIV infections of the cat offer a unique opportunity to evaluate TRIMCyp-based approaches to genetic therapy for HIV infection and the treatment of AIDS.

引用

页码：8980 / 8985

页数：6

共 40 条

[31] Target cell cyclophilin a modulates human immunodeficiency virus type 1 infectivity [J].

Sokolskaja, E ;

Sayah, DM ;

Luban, J .

JOURNAL OF VIROLOGY, 2004, 78 (23) :12800-12808

[32] Specific recognition and accelerated uncoating of retroviral capsids by the TRIM5α restriction factor [J].

Stremlau, M ;

Perron, M ;

Lee, M ;

Li, Y ;

Song, B ;

Javanbakht, H ;

Diaz-Griffero, F ;

Anderson, DJ ;

Sundquist, WI ;

Sodroski, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (14) :5514-5519

[33] The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeys [J].

Stremlau, M ;

Owens, CM ;

Perron, MJ ;

Kiessling, M ;

Autissier, P ;

Sodroski, J .

NATURE, 2004, 427 (6977) :848-853

[34] FUNCTIONAL ASSOCIATION OF CYCLOPHILIN-A WITH HIV-1 VIRIONS [J].

THALI, M ;

BUKOVSKY, A ;

KONDO, E ;

ROSENWIRTH, B ;

WALSH, CT ;

SODROSKI, J ;

GOTTLINGER, HG .

NATURE, 1994, 372 (6504) :363-365

[35] Independent genesis of chimeric TRIMS-cyclophilin proteins in two primate species [J].

Virgen, Cesar A. ;

Kratovac, Zerina ;

Bieniasz, Paul D. ;

Hatziioannou, Theodora .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (09) :3563-3568

[36] FIV infection of the domestic cat: An animal model for AIDS [J].

Willett, BJ ;

Flynn, JN ;

Hosie, MJ .

IMMUNOLOGY TODAY, 1997, 18 (04) :182-189

[37] Independent evolution of an antiviral TRIMCyp in rhesus macaques [J].

Wilson, Sam J. ;

Webb, Benjamin L. J. ;

Ylinen, Laura M. J. ;

Verschoor, Ernst ;

Heeney, Jonathan L. ;

Towers, Greg J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (09) :3557-3562

[38] Proteasome inhibitors uncouple rhesus TRIM5α restriction of HIV-1 reverse transcription and infection [J].

Wu, Xiaolu ;

Anderson, Jenny L. ;

Campbell, Edward M. ;

Joseph, Ajith M. ;

Hope, Thomas J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (19) :7465-7470

[39] Ubiquitination of E3 ubiquitin ligase TRIM5α and its potential role [J].

Yamauchi, Keiko ;

Wada, Keiji ;

Tanji, Kunikazu ;

Tanaka, Makoto ;

Kamitani, Tetsu .

FEBS JOURNAL, 2008, 275 (07) :1540-1555

[40] Molecular recognition in the HIV-1 capsid/cyclophilin a complex [J].

Yoo, SH ;

Myszka, DG ;

Yeh, CY ;

McMurray, M ;

Hill, CP ;

Sundquist, WI .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 269 (05) :780-795

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