Regulation of polyphenol production in Vitis vinifera cell suspension cultures by sugars

被引：99

作者：

Larronde, F ^{[1
]}

Krisa, S ^{[1
]}

Decendit, A ^{[1
]}

Chèze, C ^{[1
]}

Mérillon, JM ^{[1
]}

机构：

[1] Univ Bordeaux 2, Grp Etud Subst Nat Interet Therapeut, EA 491, Fac Pharm, F-33000 Bordeaux, France

来源：

PLANT CELL REPORTS | 1998年 / 17卷 / 12期

关键词：

Vitis vinifera; cell suspension culture; stilbenes; anthocyanins; sugars;

D O I：

10.1007/s002990050515

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Sucrose was found to modulate polyphenol accumulation in Vitis vinifera cell cultures. The production of anthocyanins increased 12-fold after addition of 0.15 M sucrose, while that of stilbenes was only slightly affected. Sucrose did not play a physical role because metabolic sugars were required thr the induction of polyphenol accumulation. Indeed. the polyols, mannitol and sorbitol, had no effect on this accumulation. We established a model system to investigate the mechanism of sucrose regulation of poly phenol production without inhibition of grape cell growth. After addition of sucrose to the culture medium, the major sugars accumulated in grape cells were glucose and fructose, reaching 40% of the dry weight. The increase in the level of these hexoses closely coincided with the increase in anthocyanin accumulation in grape cells.

引用

页码：946 / 950

页数：5

共 27 条

[1] Analysis of the expression of anthocyanin pathway genes in developing Vitis vinifera L cv Shiraz grape berries and the implications for pathway regulation [J].