Initial analysis of 750 MHz NMR spectra of selectively N-15-G,U labelled E-coli 5S rRNA

被引：14

作者：

Grune, M

Gorlach, M

Soskic, V

Klussmann, S

Bald, R

Furste, JP

Erdmann, VA

Brown, LR

机构：

[1] INST MOL BIOTECHNOL EV, D-07708 JENA, GERMANY

[2] FREE UNIV BERLIN, INST BIOCHEM, D-14195 BERLIN, GERMANY

来源：

FEBS LETTERS | 1996年 / 385卷 / 1-2期

关键词：

NMR; N-15; labelling; ribosome; 5S rRNA; E-domain; E-coli;

D O I：

10.1016/0014-5793(96)00361-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The overall folding of an RNA molecule is reflected in its base pairing pattern. The identification of that pattern provides a first step towards the determination of the structure of an RNA molecule. We show that the application of heteronuclear NMR methods at 750 MHz to E. coli 5S rRNA (120 nucleotides) selectively labelled with N-15 in guanine and uridine allows observation of base pairing patterns for a larger RNA molecule. We also present evidence that the fold of the E-domain of the 5S rRNA (nt 79-97) as a contiguous part of the 5S rRNA and as an isolated molecule is virtually the same.

引用

收藏

页码：114 / 118

页数：5

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