Initial analysis of 750 MHz NMR spectra of selectively N-15-G,U labelled E-coli 5S rRNA

被引：14

作者：

Grune, M

Gorlach, M

Soskic, V

Klussmann, S

Bald, R

Furste, JP

Erdmann, VA

Brown, LR

机构：

[1] INST MOL BIOTECHNOL EV, D-07708 JENA, GERMANY

[2] FREE UNIV BERLIN, INST BIOCHEM, D-14195 BERLIN, GERMANY

来源：

FEBS LETTERS | 1996年 / 385卷 / 1-2期

关键词：

NMR; N-15; labelling; ribosome; 5S rRNA; E-domain; E-coli;

D O I：

10.1016/0014-5793(96)00361-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The overall folding of an RNA molecule is reflected in its base pairing pattern. The identification of that pattern provides a first step towards the determination of the structure of an RNA molecule. We show that the application of heteronuclear NMR methods at 750 MHz to E. coli 5S rRNA (120 nucleotides) selectively labelled with N-15 in guanine and uridine allows observation of base pairing patterns for a larger RNA molecule. We also present evidence that the fold of the E-domain of the 5S rRNA (nt 79-97) as a contiguous part of the 5S rRNA and as an isolated molecule is virtually the same.

引用

收藏

页码：114 / 118

页数：5

相关论文

共 41 条

[11] HETERONUCLEAR TECHNIQUES IN NMR-STUDIES OF RNA AND DNA [J].

DIECKMANN, T ;

FEIGON, J .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1994, 4 (05) :745-749

[12] STEM-LOOP-IV OF 5S RIBOSOMAL-RNA LIES CLOSE TO THE PEPTIDYLTRANSFERASE CENTER [J].

DONTSOVA, O ;

TISHKOV, V ;

DOKUDOVSKAYA, S ;

BOGDANOV, A ;

DORING, T ;

RINKEAPPEL, J ;

THAMM, S ;

GREUER, B ;

BRIMACOMBE, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (10) :4125-4129

[13] STRUCTURE AND FUNCTION OF 5S RNA - ROLE OF 3' TERMINUS IN 5S RNA FUNCTION [J].

ERDMANN, VA ;

SPRINZL, M ;

DOBERER, HG .

MOLECULAR AND GENERAL GENETICS, 1972, 114 (02) :89-&

[14]

GESTELAND RF, 1993, COLD SPRING HARBOR M, V24

[15] EFFECTS OF MUTATION ON THE DOWNFIELD PROTON NUCLEAR-MAGNETIC-RESONANCE SPECTRUM OF THE 5S RNA OF ESCHERICHIA-COLI [J].

GEWIRTH, DT ;

MOORE, PB .

BIOCHEMISTRY, 1987, 26 (18) :5657-5665

[16] SECONDARY STRUCTURE OF 5S RNA - NMR EXPERIMENTS ON RNA MOLECULES PARTIALLY LABELED WITH N-15 [J].

GEWIRTH, DT ;

ABO, SR ;

LEONTIS, NB ;

MOORE, PB .

BIOCHEMISTRY, 1987, 26 (16) :5213-5220

[17]

GORINGER HU, 1984, J BIOL CHEM, V259, P491

[18] OMPT ENCODES THE ESCHERICHIA-COLI OUTER-MEMBRANE PROTEASE THAT CLEAVES T7-RNA POLYMERASE DURING PURIFICATION [J].

GRODBERG, J ;

DUNN, JJ .

JOURNAL OF BACTERIOLOGY, 1988, 170 (03) :1245-1253

[19] THE IMPORTANCE OF NOT SATURATING H2O IN PROTEIN NMR - APPLICATION TO SENSITIVITY ENHANCEMENT AND NOE MEASUREMENTS [J].

GRZESIEK, S ;

BAX, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (26) :12593-12594

[20] RIBOSOMAL PROTEINS OF ESCHERICHIA COLI .I. PURIFICATION OF 30S RIBOSOMAL PROTEINS [J].

HARDY, SJS ;

KURLAND, CG ;

VOYNOW, P ;

MORA, G .

BIOCHEMISTRY, 1969, 8 (07) :2897-&

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