Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray

被引：62

作者：

Khadijah, S

Neo, SY

Hossain, MS

Miller, LD

Mathavan, S

Kwang, J

机构：

[1] Natl Univ Singapore, Temasek Life Sci Lav, Singapore 117604, Singapore

[2] Genome Inst Singapore, Singapore 117528, Singapore

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 18期

关键词：

D O I：

10.1128/JVI.77.18.10162-10167.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.

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收藏

页码：10162 / 10167

页数：6

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