Glucocorticoid-induced maturation of glycoprotein galactosylation and fucosylation processes in the rat small intestine

被引:16
作者
Biol-N'garagba, MC
Niepceron, E
Mathian, B
Louisot, P
机构
[1] CNRS, Unite INSERM, Fac Med, Dept Biochim, F-69600 Oullins, France
[2] Ctr Hosp Lyon Sud, Hormonal Lab, F-69310 Pierre Benite, France
关键词
glycoprotein; galactosyltransferase; alpha-1,2-fucosyltransferase; small intestine; postnatal development; hydrocortisone;
D O I
10.1016/S0960-0760(03)00062-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We determined the role of glucocorticoids in the maturation of glycoprotein galactosylation and fucosylation processes in the rat small intestine during postnatal development. Treatment of suckling rats with hydrocortisone (HC) increased activities of an O-glycan: galactosyltransferase, and of an alpha-1,2-fucosyltransferase, through transcriptional regulation of the FTB gene. The activities of a fucosyltransferase inhibitor and of the enzymes responsible for the synthesis and degradation of GDP-fucose were unaffected by the treatment, whereas a fall in the activity Of alpha-L-fucosidase was observed. These changes were accompanied by the precocious appearance of alpha-1,2-fucose residues in complex glycan chains of brush-border membrane glycoproteins that normally appear after weaning, and with a trend to increase in alpha-1,2-fucose residues in mucins. Thus, treatment of suckling rats with hydrocortisone speeds up the maturation of glycoprotein galactosylation and fucosylation processes in the small intestine. The delayed increase in glucocorticoid levels induced by prolonged nursing, or the suppression of glucocorticoids by adrenalectomy (AD) before the normal rise in the hormone, both induced a delay in the increases in activities of the O-glycan: galactosyltransferase and alpha-1,2-fucosyltransferase observed normally after glucocorticoid enhancement. Thus, glucocorticoids might play at least a partial role in the maturation of glycoprotein glycosylation observed at weaning (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:411 / 422
页数:12
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