Red and blue ferritin nanomagnets by dye-labeling to the protein shell

被引：13

作者：

Fernandez, Belen

Galvez, Natividad

Sanchez, Purificacion

Morales, Julia

Santoyo, Francisco

Cuesta, Rafael

Dominguez-Vera, Jose M. ^{[1
]}

机构：

[1] Univ Granada, Inst Biotechnol, Dept Quim Inorgan, Fac Ciencias, E-18071 Granada, Spain

[2] Univ Granada, Inst Biotechnol, Dept Quim Organ, Fac Ciencias, E-18071 Granada, Spain

[3] EPS Linares, Dept Quim Inorgan & Organ, Linares 23700, Spain

来源：

INORGANICA CHIMICA ACTA | 2007年 / 360卷 / 13期

关键词：

nanomagnet; ferritin; metallic nanoparticles; organic dye;

D O I：

10.1016/j.ica.2007.02.018

中图分类号：

O61 [无机化学];

学科分类号：

070301 [无机化学]; 081704 [应用化学];

摘要：

We report a flexible route to chemically modify the ferritin nanomagnet. Two dye-labeled ferritins (red- and blue-ferritin) have been prepared by covalently coupling the derivatives of the reactive orange 16 and Remazol brilliant blue R, respectively, to the ferritin surface lysine residues. The study of the particles by transmission electron microscopy showed that the native iron core ferritin remains intact after chemical functionalization of the protein shell. Likewise, magnetic measurements showed that the superparamagnetic properties of the iron core are preserved. (c) 2007 Elsevier B.V. All rights reserved.

引用

收藏

页码：3951 / 3954

页数：4

相关论文

共 22 条

[1]

Fluorescence-modified superparamagnetic nanoparticles:: Intracellular uptake and use in cellular imaging [J].

Bertorelle, Franck ;

Wilhelm, Claire ;

Roger, Jacky ;

Gazeau, Florence ;

Menager, Christine ;

Cabuil, Valerie .

LANGMUIR, 2006, 22 (12) :5385-5391

[2]

Magnetic Langmuir-Blodgett films of ferritin with different iron contents [J].

Clemente-Leon, Miguel ;

Coronado, Eugenio ;

Soriano-Portillo, Alejandra ;

Colacio, Enrique ;

Dominguez-Vera, Jose M. ;

Galvez, Natividad ;

Madueno, Rafael ;

Martin-Romero, Maria T. .

LANGMUIR, 2006, 22 (16) :6993-7000

[3]

Self-assembled magnetic matrices for DNA separation chips [J].

Doyle, PS ;

Bibette, J ;

Bancaud, A ;

Viovy, JL .

SCIENCE, 2002, 295 (5563) :2237-2237

[4]

Non-Langevin behaviour of the uncompensated magnetization in nanoparticles of artificial ferritin [J].

Gilles, C ;

Bonville, P ;

Wong, KKW ;

Mann, S .

EUROPEAN PHYSICAL JOURNAL B, 2000, 17 (03) :417-427

[5]

Using biofunctional magnetic nanoparticles to capture vancomycin-resistant enterococci and other gram-positive bacteria at ultralow concentration [J].

Gu, HW ;

Ho, PL ;

Tsang, KWT ;

Wang, L ;

Xu, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (51) :15702-15703

[6]

Organic-dye-coupted magnetic nanoparticles encaged inside thermoresponsive PNIPAM microcapsutes [J].

Guo, J ;

Yang, WL ;

Deng, YH ;

Wang, CC ;

Fu, SK .

SMALL, 2005, 1 (07) :737-743

[7]

Characterization and MRI study of surfactant-coated superparamagnetic nanoparticles administered into the rat brain [J].

Kim, DK ;

Zhang, Y ;

Kehr, J ;

Klason, T ;

Bjelke, B ;

Muhammed, M .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2001, 225 (1-2) :256-261

[8]

KOENIG SH, 1996, ACAD RADIOL, V3, P273

[9]

Magnetic iron oxide nanoparticles for biorecognition: Evaluation of surface coverage and activity [J].

Koh, I ;

Wang, X ;

Varughese, B ;

Isaacs, L ;

Ehrman, SH ;

English, DS .

JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (04) :1553-1558

[10]

Multifunctional nano-architecture for biomedical applications [J].

Ma, DL ;

Guan, JW ;

Normandin, F ;

Dénommée, S ;

Enright, G ;

Veres, T ;

Simard, B .

CHEMISTRY OF MATERIALS, 2006, 18 (07) :1920-1927