Determination of 24(R)-pseudoginsenoside F11 in North American ginseng using high performance liquid chromatography with evaporative light scattering detection

被引：49

作者：

Li, WK ^{[1
]}

Fitzloff, JF ^{[1
]}

机构：

[1] Univ Illinois, Program Collaborat Res Pharmaceut Sci, Dept Med Chem & Pharmacognosy, Coll Pharm,FFH Core analyt Lab, Chicago, IL 60612 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2001年 / 25卷 / 02期

关键词：

24(R)-pseudoginsenoside F-11; North American ginseng; high performance liquid chromatography evaporative light scattering detection;

D O I：

10.1016/S0731-7085(00)00494-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A gradient liquid chromatographic method with evaporative light scattering detection (ELSD) for the determination of 24(R)-pseudoginsenoside F-11 in North American ginseng is described. Samples are analyzed by means of a reverse-phase column (Waters Spherisorb ODS-2, C-18) using acetonitrile and water under gradient conditions as the mobile phase over 20 min. The evaporative light scattering detector (ELSD) used, was set at an evaporating temperature of 35 degreesC and nitrogen gas pressure of 3.4 bar. The detection limit (S/N > 5) of 24(R)-pseudoginsenoside F-11 is 53 ng on column. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：257 / 265

页数：9

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