C/EBPδ and C/EBPγ bind the CCAAT-box in the human β-globin promoter and modulate the activity of the CACC-box binding protein, EKLF

被引：15

作者：

Gordon, CT ^{[1
]}

Fox, VJ ^{[1
]}

Najdovska, S ^{[1
]}

Perkins, AC ^{[1
]}

机构：

[1] Univ Queensland, Inst Mol Biosci, St Lucia, Qld 4072, Australia

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2005年 / 1729卷 / 01期

基金：

英国医学研究理事会;

关键词：

CCAAT-box; C/EBP delta; C/EBP gamma; EKLF; beta-globin promoter; luciferase assay;

D O I：

10.1016/j.bbaexp.2005.03.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Developmental- and tissue-specific expression of globin genes is mediated by a few key elements within the proximal promoter of each gene. DNA-binding assays previously identified NF-Y, GATA-1, C/EBP beta and C/EBP gamma as candidate regulators of beta-globin transcription via the CCAAT-box, a promoter element situated between CACC- and TATA-boxes. We have identified C/EBP delta as an additional beta-globin CCAAT-box binding protein. In reporter assays, we show that C/EBP delta can co-operate with EKLF, a CACC-box binding protein, to activate the beta-globin promoter, whereas C/EBP gamma inhibits the transcriptional activity of EKLF in this assay. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：74 / 80

页数：7

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