Determination of midazolam and its metabolites in serum microsamples by high-performance liquid chromatography and its application to pharmacokinetics in rats

被引：17

作者：

Ma, F ^{[1
]}

Lau, CE ^{[1
]}

机构：

[1] RUTGERS STATE UNIV,DEPT PSYCHOL,NEW BRUNSWICK,NJ 08903

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 682卷 / 01期

关键词：

midazolam;

D O I：

10.1016/0378-4347(96)00069-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A single-solvent extraction step high-performance liquid chromatographic method is described for quantitating midazolam and its two hydroxy metabolites in rat serum microsamples (50 mu l). The separation used a 2 mm I.D. reversed-phase Symmetry C-18 column with an isocratic mobile phase consisting of methanol-acetonitrile-14.9 mM sodium acetate in water at pH 3.0 (10:23:67, v/v). The detection limit was 10 ng/ml for all the compounds using an ultraviolet detector operated at 230 nm. The method was used to study the pharmacokinetics of midazolam after an intravenous bolus dose (0.75 mg/kg).

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页码：109 / 113

页数：5

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