Multidimensional CP-MAS 13C NMR of uniformly enriched chlorophyll

被引:43
作者
van Rossum, BJ
Boender, GJ
Mulder, FM
Raap, J
Balaban, TS
Holzwarth, A
Schaffner, K
Prytulla, S
Oschkinat, H
de Groot, HJM
机构
[1] Leiden State Univ, Gorlaeus Labs, Leiden Inst Chem, NL-2300 RA Leiden, Netherlands
[2] Max Planck Inst Strahlenchem, D-45470 Mulheim, Germany
[3] European Mol Biol Lab, D-69117 Heidelberg, Germany
关键词
antenna; photosynthesis; MAS NMR; isotope labelling; chlorophyll;
D O I
10.1016/S1386-1425(98)00067-5
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
The progress toward structure refinement of solid-type uniformly C-13 enriched ([U-C-13]) chlorophyll-containing biological preparations is summarised. Solid state carbon chemical shifts of aggregated [U-C-13] bacteriochlorophyll (BChl) c in intact chlorosomes of Chlorobium tepidum and in [U-C-13] BChl c aggregates were determined by the application of homonuclear (C-13-C-13) magic angle spinning (MAS) NMR dipolar correlation spectroscopy. It was found that the arrangement of BChl c molecules in the chlorosomes and in the aggregates is highly similar, which provides convincing evidence that self-organisation of the BChl c is the main mechanism to support the structure of the chlorosomes. Additionally, high held 2-D (H-1-C-13) and 3-D (H-1-C-13-C-13) dipolar correlation spectroscopy was applied to determine solid state proton chemical shifts of aggregated [U-C-13] BChl c in intact chlorosomes. From the high-field assignments, evidence is found for the existence of at least two well-defined interstack arrangements. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:1167 / 1176
页数:10
相关论文
共 37 条
[1]   CP-MAS C-13-NMR DIPOLAR CORRELATION SPECTROSCOPY OF C-13-ENRICHED CHLOROSOMES AND ISOLATED BACTERIOCHLOROPHYLL-C AGGREGATES OF CHLOROBIUM-TEPIDUM - THE SELF-ORGANIZATION OF PIGMENTS IS THE MAIN STRUCTURAL FEATURE OF CHLOROSOMES [J].
BALABAN, TS ;
HOLZWARTH, AR ;
SCHAFFNER, K ;
BOENDER, GJ ;
DEGROOT, HJM .
BIOCHEMISTRY, 1995, 34 (46) :15259-15266
[2]   BROAD-BAND POLARIZATION-TRANSFER EXPERIMENTS FOR ROTATING SOLIDS [J].
BALDUS, M ;
TOMASELLI, M ;
MEIER, BH ;
ERNST, RR .
CHEMICAL PHYSICS LETTERS, 1994, 230 (4-5) :329-336
[3]  
BENNET AE, 1995, J CHEM PHYS, V1023, P6951
[4]   MAS NMR STRUCTURE REFINEMENT OF UNIFORMLY C-13 ENRICHED CHLOROPHYLL-A WATER AGGREGATES WITH 2D DIPOLAR CORRELATION SPECTROSCOPY [J].
BOENDER, GJ ;
RAAP, J ;
PRYTULLA, S ;
OSCHKINAT, H ;
DEGROOT, HJM .
CHEMICAL PHYSICS LETTERS, 1995, 237 (5-6) :502-508
[5]  
BOENDER GJ, 1995, PHOTOSYNTHESIS LIGHT, V1, P347
[6]  
BOENDER GJ, 1996, THESIS U LEIDEN LEID
[7]  
BYSTROVA MI, 1979, MOL BIOL+, V13, P440
[8]   C-13 MAGIC ANGLE SPINNING NMR EVIDENCE FOR A 15,15'-CIS CONFIGURATION OF THE SPHEROIDENE IN THE RHODOBACTER-SPHAEROIDES PHOTOSYNTHETIC REACTION CENTER [J].
DEGROOT, HJM ;
GEBHARD, R ;
VANDERHOEF, I ;
HOFF, AJ ;
LUGTENBURG, J ;
VIOLETTE, CA ;
FRANK, HA .
BIOCHEMISTRY, 1992, 31 (49) :12446-12450
[9]  
DEGROOT HJM, 1996, ADV PHOTOSYNTH, V3, P299
[10]   Characterization of pheophytin ground states in Rhodobacter sphaeroides R26 photosynthetic reaction centers from multispin pheophytin enrichment and 2-D C-13 MAS NMR dipolar correlation spectroscopy [J].
EgorovaZachernyuk, TA ;
vanRossum, B ;
Boender, GJ ;
Franken, E ;
Ashurst, J ;
Raap, J ;
Gast, P ;
Hoff, AJ ;
Oschkinat, H ;
deGroot, HJM .
BIOCHEMISTRY, 1997, 36 (24) :7513-7519