Collagen-induced arthritis

被引：1095

作者：

Brand, David D. ^{[1
,2
,3
,4
]}

Latham, Kary A. ^{[1
,2
,3
,4
]}

Rosloniec, Edward F. ^{[1
,2
,3
,4
]}

机构：

[1] Vet Adm Med Ctr, Res Serv, Memphis, TN 38104 USA

[2] Univ Tennessee, Hlth Sci Ctr, Dept Med, Memphis, TN 38163 USA

[3] Univ Tennessee, Hlth Sci Ctr, Dept Pathol, Memphis, TN 38163 USA

[4] Univ Tennessee, Hlth Sci Ctr, Dept Mol Sci, Memphis, TN 38163 USA

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 05期

关键词：

D O I：

10.1038/nprot.2007.173

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The collagen-induced arthritis (CIA) mouse model is the most commonly studied autoimmune model of rheumatoid arthritis. Autoimmune arthritis is induced in this model by immunization with an emulsion of complete Freund's adjuvant and type II collagen (CII). This protocol describes the steps necessary for acquisition, handling and preparation of CII, as well as selection of mouse strains, proper immunization technique and evaluation of the arthritis incidence and severity. Typically, the first signs of arthritis appear in this model 21-28 days after immunization, and identification of the arthritic limbs is not difficult. Using the protocol described, the investigator should be able to reproducibly induce a high incidence of CIA in various strains of genetically susceptible mice as well as learn how to critically evaluate the pathology of the disease. The total time for the preparation of reagents and the immunization of ten mice is about 1.5 h.

引用

页码：1269 / 1275

页数：7

共 35 条

[11] IFNγ deficient C57BL/6 (H-2b) mice develop collagen induced arthritis with predominant usage of T cell receptor Vβ6 and Vβ8 in arthritic joints [J].