Fuel ethanol production from corn fiber - Current status and technical prospects

被引:55
作者
Saha, BC [1 ]
Dien, BS [1 ]
Bothast, RJ [1 ]
机构
[1] ARS, Fermentat Biochem Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA
关键词
fuel ethanol; corn fiber; pretreatment; enzymatic saccharification; fermentation;
D O I
10.1007/BF02920129
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Corn fiber, which consists of about 20% starch, 14% cellulose, and 35% hemicellulose, has the potential to serve as a low cost feedstock for production of fuel ethanol. Currently, the use of corn fiber to produce fuel ethanol faces significant technical and economic challenges. Its success depends largely on the development of environmentally friendly pretreatment procedures, highly effective enzyme systems for conversion of pretreated corn fiber to fermentable sugars, and efficient microorganisms to convert multiple sugars to ethanol. Several promising pretreatment and enzymatic processes for conversion of corn fiber cellulose, hemicellulose, and remaining starch to fermentable sugars were evaluated. These hydrolyzates were then examined for ethanol production in bioreactors, using genetically modified bacteria and yeast. Several novel enzymes were also developed for use in pretreated corn fiber saccharification.
引用
收藏
页码:115 / 125
页数:11
相关论文
共 36 条
[11]   Stabilization of pet operon plasmids and ethanol production in Escherichia coli strains lacking lactate dehydrogenase and pyruvate formate-lyase activities [J].
Hespell, RB ;
Wyckoff, H ;
Dien, BS ;
Bothast, RJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (12) :4594-4597
[12]   Hydrolysis by commercial enzyme mixtures of AFEX-treated corn fiber and isolated xylans' [J].
Hespell, RB ;
OBryan, PJ ;
Moniruzzaman, M ;
Bothast, RJ .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1997, 62 (01) :87-97
[13]  
HO NWY, 1995, Patent No. 13362
[14]   GENETIC-ENGINEERING OF ETHANOL-PRODUCTION IN ESCHERICHIA-COLI [J].
INGRAM, LO ;
CONWAY, T ;
CLARK, DP ;
SEWELL, GW ;
PRESTON, JF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (10) :2420-2425
[15]   Saccharification of corn fiber using enzymes from Aureobasidium sp strain NRRL Y-2311-1 [J].
Leathers, TD ;
Gupta, SC .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1996, 59 (03) :337-347
[16]   PURIFICATION AND CHARACTERIZATION OF AN ALPHA-L-ARABINOFURANOSIDASE FROM CLOSTRIDIUM-ACETOBUTYLICUM ATCC-824 [J].
LEE, SF ;
FORSBERG, CW .
CANADIAN JOURNAL OF MICROBIOLOGY, 1987, 33 (11) :1011-1016
[17]  
LINDSAY SE, 1995, APPL MICROBIOL BIOT, V43, P70, DOI [10.1007/s002530050372, 10.1007/BF00170625]
[18]   MUTANTS OF ESCHERICHIA-COLI DEFICIENT IN THE FERMENTATIVE LACTATE-DEHYDROGENASE [J].
MATJAN, F ;
ALAM, KY ;
CLARK, DP .
JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :342-348
[19]   ARABINOSE UTILIZATION BY XYLOSE-FERMENTING YEASTS AND FUNGI [J].
MCMILLAN, JD ;
BOYNTON, BL .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1994, 45-6 :569-584
[20]   Ethanol production from AFEX pretreated corn fiber by recombinant bacteria [J].
Moniruzzaman, M ;
Dien, BS ;
Ferrer, B ;
Hespell, RB ;
Dale, BE ;
Ingram, LO ;
Bothast, RJ .
BIOTECHNOLOGY LETTERS, 1996, 18 (08) :985-990