An alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue

被引：57

作者：

Coura, R ^{[1
]}

Prolla, JC ^{[1
]}

Meurer, L ^{[1
]}

Ashton-Prolla, P ^{[1
]}

机构：

[1] Univ Fed Rio Grande Sul, Genet & Mol Biol Postgrad Program, Med Genet Serv, Hosp Clin Porto Alegre, BR-90035003 Rio Grande Do Sul, Brazil

来源：

JOURNAL OF CLINICAL PATHOLOGY | 2005年 / 58卷 / 08期

关键词：

D O I：

10.1136/jcp.2004.021352

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Background: DNA extraction from paraffin wax embedded tissue requires special protocols, and most described methods report an amplification success rate of 60 - 80%. Aims: To propose a simple and inexpensive protocol consisting of xylene/ ethanol dewaxing, followed by a kit based extraction. Method: Xylene/ ethanol dewaxing was followed by a long rehydration step and a kit based DNA extraction step. Results: This method produced a 100% amplification success rate for fragments of 121 to 227 bp for tamponated formalin fixed paraffin wax embedded tissue. Conclusion: This cost effective and non- laborious protocol can successfully extract DNA from tamponated formalin fixed paraffin wax embedded tissue and should facilitate the molecular analysis of a large number of archival specimens in retrospective studies.

引用

页码：894 / 895

页数：2

共 7 条

[1] Comparison of methods for DNA extraction from paraffin-embedded tissues and buccal cells [J].