Selection of deoxyribozyme ligases that catalyze the formation of an unnatural internucleotide linkage

被引：21

作者：

Levy, M ^{[1
]}

Ellington, AD ^{[1
]}

机构：

[1] Univ Texas, Inst Cellular & Mol Biol, Dept Chem & Biochem, Austin, TX 78712 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2001年 / 9卷 / 10期

关键词：

D O I：

10.1016/S0968-0896(01)00033-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chemical ligation of DNA molecules can be mediated by terminal phosphorothioate displacement of a 5' iodine. We have selected deoxyribozymes that can catalyze the formation of such phosphorothioester internucleotide linkages. The selected deoxyribozymes enhance the rate of ligation in part through the provision of a template that aligns the ligation junction and do not appear to require metal ions for catalysis. (C) 2001 Published by Elsevier Science Ltd.

引用

页码：2581 / 2587

页数：7

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