Valproate induces apoptosis by inducing accumulation of neutral lipids which was prevented by disruption of the SIR2 gene in Saccharomyces cerevisiae

被引：18

作者：

Sun, Qi

Bi, Lifu

Su, XiuLan

Tsurugi, Kunio

Mitsui, Kazuhiro

机构：

[1] Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Biochem 2, Yamanashi 4093898, Japan

[2] Inner Mongolia Med Coll, Dept Immunol, Inner Mongolia 010059, Peoples R China

来源：

FEBS LETTERS | 2007年 / 581卷 / 21期

关键词：

budding yeast; apoptosis; valproate; lipid; accumulation; SIR2;

D O I：

10.1016/j.febslet.2007.07.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the participation of HDACs in VPA induced apoptosis in Saccharomyces cerevisiae. VPA (20 mM) induced apoptosis in several HDAC mutants, including PRD3 and HDA1-disrupted cells and SIR2 over expressing cells, as well as in wild-type cells but not SIR2-disrupted cells. Intracellular reactive oxygen species and neutral lipid content increased markedly in all kinds of HDAC mutant cells tested except for SIR2-disrupted cells. Thus, these results suggest that 20 mM VPA induces neutral lipid accumulation and apoptosis-like features in S. cerevisiae, and that VPA-induced apoptosis was evaded by deletion of SIR2. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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收藏

页码：3991 / 3995

页数：5

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