Stable-isotope probing of nucleic acids: a window to the function of uncultured microorganisms

被引：174

作者：

Radajewski, S ^{[1
]}

McDonald, IR ^{[1
]}

Murrell, JC ^{[1
]}

机构：

[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2003年 / 14卷 / 03期

关键词：

D O I：

10.1016/S0958-1669(03)00064-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phylogeny based on ribosomal RNA sequences alone is rarely a reliable indicator of microbial function. To circumvent this problem, nucleic acid based techniques have been developed that exploit the physical properties of stable isotopes to study microbially mediated processes within complex environmental samples. Investigations using labelled substrates, or which detect variations in the natural abundance of isotopes, have thus revealed the metabolic function of microorganisms without the need to isolate them in culture.

引用

页码：296 / 302

页数：7

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