A simple test for the detection of KPC and metallo-β-lactamase carbapenemase-producing Pseudomonas aeruginosa isolates with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin

被引：34

作者：

Pasteran, F. ^{[1
]}

Veliz, O. ^{[1
]}

Faccone, D. ^{[1
]}

Guerriero, L. ^{[1
]}

Rapoport, M. ^{[1
]}

Mendez, T. ^{[1
]}

Corso, A. ^{[1
]}

机构：

[1] ANLIS Dr Carlos G Malbra, INEI, Serv Antimicrobianos, Dept Bacteriol,Minist Salud & Ambiente, Buenos Aires, DF, Argentina

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2011年 / 17卷 / 09期

关键词：

3-Aminophenylboronic acid; carbapenemase; cloxacillin; dipicolinic acid; Pseudomonas aeruginosa; CLASS-A CARBAPENEMASE; KLEBSIELLA-PNEUMONIAE; ENTEROBACTERIACEAE; RESISTANCE;

D O I：

10.1111/j.1469-0691.2011.03585.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We evaluated the ability of the combination disk test (CDT) and the Modified Hodge Test (MHT) to discriminate between various carbapenemase-producing Pseudomonas aeruginosa isolates (KPC, n = 36; metallo-beta-lactamase (MBL), n = 38) and carbapenemase non-producers (n = 75). For the CDT, the optimal inhibitor concentrations and cut-off values were: 600 mu g of 3-aminophenylboronic acid (APB) per disk (an increment of >= 4 mm), 1000 mu g of dipicolinic acid (DPA) per disk (an increment of 5 mm) and 3000 mu g of cloxacillin per disk (an increment of 3 mm). APB had excellent sensitivity (97%) and specificity (97%) for the detection of KPC enzymes. DPA detected MBL enzymes with a sensitivity and specificity of 97% and 81%, respectively. The MHT resulted in a low sensitivity (78%) and specificity (57%). The CDT could be very useful in daily practice to provide fast and reliable detection of KPC and MBL carbapenemases among P. aeruginosa isolates.

引用

页码：1438 / 1441

页数：5

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