Detection of extended-spectrum β-lactamases in clinical isolates of Pseudomonas aeruginosa

被引:105
作者
Jiang, Xiaofei [1 ]
Zhang, Zhe [1 ]
Li, Min [1 ]
Zhou, Danqiu [1 ]
Ruan, Feiyi [1 ]
Lu, Yuan [1 ]
机构
[1] Fudan Univ, Huashan Hosp, Ctr Lab Med, Shanghai Med Coll, Shanghai 200040, Peoples R China
关键词
D O I
10.1128/AAC.01511-05
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
With the occurrence of extended-spectrum beta-lactamases (ESBLs) in Pseudomonas aeruginosa being increasingly reported worldwide, there is a need for a reliable test to detect ESBLs in clinical isolates of P. aeruginosa. In our study, a total of 75 clinical isolates of P. aeruginosa were studied. Nitrocefin tests were performed to detect the P-lactamase enzyme; isoelectric focusing electrophoresis, PCR, and PCR product sequencing were designed to further characterize the contained ESBLs. Various ESBL-screening methods were designed to compare the reliabilities of detecting ESBLs in clinical isolates of P. aeruginosa whose beta-lactamases were well characterized. Thirty-four of 36 multidrug-resistant P. aeruginosa clinical isolates were positive for ESBLs. bla(VEB-3) was the most prevalent ESBL gene in P. aeruginosa in our study. Among the total of 34 isolates that were considered ESBL producers, 20 strains were positive using conventional combined disk tests and 10 strains were positive using a conventional double-disk synergy test (DDST) with amoxicillin-clavulanate, expanded-spectrum cephalosporins, aztreonam, and cefepime. Modifications of the combined disk test and DDST, which consisted of shorter distances between disks (20 mm instead of 30 mm) and the use of three different plates that contained cloxacillin (200 mu g/ml) alone, Phe-Arg beta-naphthylamide dihydrochloride (MC-207,110; 20 mu g/ml) alone, and both cloxacillin (200 mu g/ml) and MC-207,110 (20 mu g/ml) increased the sensitivity of the tests to 78.8%, 91.18%, 85.29%, and 97.06%.
引用
收藏
页码:2990 / 2995
页数:6
相关论文
共 21 条
  • [1] Functional and structural characterization of the genetic environment of an extended-spectrum β-lactamase blaVEB gene from a Pseudomonas aeruginosa isolate obtained in India
    Aubert, D
    Girlich, D
    Naas, T
    Nagarajan, S
    Nordmann, P
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (09) : 3284 - 3290
  • [2] β-lactamase-mediated resistance to extended spectrum cephalosporins among clinical isolates of Pseudomonas aeruginosa
    Ben-Mahrez, K
    Rejiba, S
    Belhadj, C
    Belhadj, O
    [J]. RESEARCH IN MICROBIOLOGY, 1999, 150 (06) : 403 - 406
  • [3] Detection of extended-spectrum β-lactamases in klebsiellae with the oxoid combination disk method
    Carter, MW
    Oakton, KJ
    Warner, M
    Livermore, DM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) : 4228 - 4232
  • [4] Detection of extended-spectrum beta-lactamase (ESBL)-producing strains by the Etest ESBL screen
    Cormican, MG
    Marshall, SA
    Jones, RN
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (08) : 1880 - 1884
  • [5] Comparison of different PCR approaches for typing of Francisella tularensis strains
    de la Puente-Redondo, VA
    del Blanco, NG
    Gutiérrez-Martín, CB
    García-Peña, FJ
    Ferri, EFR
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) : 1016 - 1022
  • [6] Laboratory detection of extended-spectrum β-lactamases (ESBLs) -: The need for a reliable, reproducible method
    Essack, SY
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2000, 37 (04) : 293 - 295
  • [7] ERIC SEQUENCES - A NOVEL FAMILY OF REPETITIVE ELEMENTS IN THE GENOMES OF ESCHERICHIA-COLI, SALMONELLA-TYPHIMURIUM AND OTHER ENTEROBACTERIA
    HULTON, CSJ
    HIGGINS, CF
    SHARP, PM
    [J]. MOLECULAR MICROBIOLOGY, 1991, 5 (04) : 825 - 834
  • [8] Detection of extended-spectrum beta-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli
    Jacoby, GA
    Han, P
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (04) : 908 - 911
  • [9] Outbreak of infection caused by Enterobacter cloacae producing the novel VEB-3 beta-lactamase in China
    Jiang, XF
    Ni, YX
    Jiang, YQ
    Yuan, FY
    Han, LZ
    Li, M
    Liu, H
    Yang, L
    Lu, Y
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (02) : 826 - 831
  • [10] [蒋晓飞 JIANG Xiaofei], 2005, [中华检验医学杂志, Chinese Journal of Laboratory Medicine], V28, P1018