Simultaneous determination of cysteine, cystine and 18 other amino acids in various matrices by high-performance liquid chromatography

被引：66

作者：

Campanella, L ^{[1
]}

Crescentini, G ^{[1
]}

Avino, P ^{[1
]}

机构：

[1] Univ Rome La Sapienza, Dept Chem, I-00185 Rome, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 833卷 / 02期

关键词：

cysteine; cystine; amino acids;

D O I：

10.1016/S0021-9673(98)01023-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An extraction protocol and a reversed-phase high-performance liquid chromatographic method for the simultaneous determination of cysteine, cystine and 18 other amino acids in biological and vegetable samples are described. Among the different methods proposed for amino acid determination, phenylisothiocyanate was used as the reagent for derivatization. Chromatograms obtained in the analysis of standard solutions and actual samples are reported, together with regression equation, correlation coefficient (>0.999 for all), limit of detection and recoveries (between 86 and 102% for all the examined matrices) for each amino acid. Practical protocol and method applications in normal patients and patients affected by different pathologies, and in algal products are discussed. (C) 1999 Published by Elsevier Science BN. All rights reserved.

引用

页码：137 / 145

页数：9

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