HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root

被引:44
作者
Han, Chao [2 ]
Shen, Yan [3 ]
Chen, Junhui [1 ]
Lee, Frank Sen-Chun [1 ]
Wang, Xiaoru [1 ]
机构
[1] First Inst Oceanog SOA, Qingdao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China
[2] Wenzhou Entry Exit Inspect & Quarantine Bur, Wenzhou 325027, Peoples R China
[3] Wenzhou Univ, Coll Chem & Mat Sci, Wenzhou 325027, Peoples R China
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 862卷 / 1-2期
关键词
fingerprint chromatogram; Pseudostellaria heterophylla (Miq.) pax; HPLC-TOF-MS; hierarchical clustering analysis; similarity analysis; cyclic peptides;
D O I
10.1016/j.jchromb.2007.11.041
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
High-performance liquid chromatographic (HPLC) was developed for fingerprint analysis of Pseudostellaria heterophylla (Miq.) Pax. Liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (LC-TOF-MS) technique was first employed to identify the components of the fingerprint. Twelve major peaks in chromatographic fingerprint were analyzed by on-line LC-TOF-MS analysis; one cyclic peptide was unequivocally identified and five cyclic peptides were tentatively assigned based on their MS data. These cyclic peptides served as the marker peaks in the HPLC fingerprints. The chromatographic fingerprints have been analyzed by similarity index calculations and hierarchical clustering analysis (HCA). The result showed that the HPLC fingerprints could be used to determine the optimal harvest time for R heterophylla (Miq.) Pax and to authenticate the species of the herb. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:125 / 131
页数:7
相关论文
共 20 条
[1]   HPLC fingerprinting and estimation of the bioactive components of Clutia richardiana L. as a potential hypoglycemic herbal tea [J].
Abourashed, EA ;
Ganzera, M ;
Khan, IA ;
Khan, S ;
Mossa, JS ;
El-Feraly, FS .
PHYTOTHERAPY RESEARCH, 2003, 17 (06) :657-660
[2]  
*BSTAT FOOD DRUG A, 2000, NOT STUD FING TRAD C
[3]  
[陈军辉 CHEN Junhui], 2005, [食品科学, Food Science], V26, P200
[4]  
Chen XG, 2006, J PHARMACEUT BIOMED, V40, P559, DOI 10.1016/j.jpba.2005.07.043
[5]   Information theory applied to chromatographic fingerprint of herbal medicine for quality control [J].
Gong, F ;
Liang, YZ ;
Xie, PS ;
Chau, FT .
JOURNAL OF CHROMATOGRAPHY A, 2003, 1002 (1-2) :25-40
[6]   Isolation and purification of Pseudostellarin B (cyclic peptide) from Pseudostellaria heterophylla (Miq.) Pax by high-speed counter-current chromatography [J].
Han, Chao ;
Chen, Junhui ;
Liu, Jie ;
Lee, Frank Sen-Chun ;
Wang, Xiaoru .
TALANTA, 2007, 71 (02) :801-805
[7]   Fingerprint chromatogram analysis of Pseudostellaria heterophylla (Miq.) Pax root by high performance liquid chromatography [J].
Han, Chao ;
Chen, Junhui ;
Chen, Bo ;
Lee, Frank Sen-Chun ;
Wang, Xiaoru .
JOURNAL OF SEPARATION SCIENCE, 2006, 29 (14) :2197-2202
[8]  
LI SH, 2001, MED MAT MED RES, V12, P199
[9]   PSEUDOSTELLARIN-G, A NEW TYROSINASE INHIBITORY CYCLIC OCTAPEPTIDE FROM PSEUDOSTELLARIA-HETEROPHYLLA [J].
MORITA, H ;
KOBATA, H ;
TAKEYA, K ;
ITOKAWA, H .
TETRAHEDRON LETTERS, 1994, 35 (21) :3563-3564
[10]   PSEUDOSTELLARINS D-F, NEW TYROSINASE INHIBITORY CYCLIC-PEPTIDES FROM PSEUDOSTELLARIA-HETEROPHYLLA [J].
MORITA, H ;
KAYASHITA, T ;
KOBATA, H ;
GONDA, A ;
TAKEYA, K ;
ITOKAWA, H .
TETRAHEDRON, 1994, 50 (33) :9975-9982