Over-expression of mammalian sialidase NEU3 reduces Newcastle disease virus entry and propagation in COS7 cells

被引:8
作者
Anastasia, Luigi [1 ]
Holguera, Javier [2 ]
Bianchi, Anna [1 ]
D'Avila, Francesca [1 ]
Papini, Nadia [1 ]
Tringali, Cristina [1 ]
Monti, Eugenio [3 ]
Villar, Enrique [2 ]
Venerando, Bruno [1 ]
Munoz-Barroso, Isabel [2 ]
Tettainanti, Guido [1 ]
机构
[1] Univ Milan, Dept Med Chem Biochem & Biotechnol, Milan, Italy
[2] Univ Salamanca, Dept Bioquim & Biol Mol, Salamanca 37007, Spain
[3] Univ Brescia, Dept Biomed & Biotechnol Sci, Brescia, Italy
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2008年 / 1780卷 / 03期
关键词
COS7; cell; NDV; sialidase NEU3; lipid raft; GD1a ganglioside;
D O I
10.1016/j.bbagen.2007.11.011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The paramyxovirus Newcastle Disease Virus (NDV) binds to sialic acid-containing glycoconjugates, sialoglycoproteins and sialoglycolipids (gangliosides) of host cell plasma membrane through its hemagglutinin-neuraminidase (sialidase) HN glycoprotein. We hypothesized that the modifications of the cell surface ganglioside pattern determined by over-expression of the mammalian plasma-membrane associated, ganglioside specific, sialidase NEU3 would affect the virus-host cell interactions. Using COS7 cells as a model system, we observed that over-expression of the murine MmNEU3 did not affect NDV binding but caused a marked reduction in NDV infection and virus propagation through cell-cell fusion. Moreover, since GD I a was greatly reduced in COS7 cells following NEU3-over-expression, we added [H-3]-labelled GDla to COS7 cells under conditions that block intralysosomal metabolic processing, and we observed a marked increase of GD I a cleavage to GM I during NDV infection, indicating a direct involvement of the virus sialidase and host cell GDla in NDV infectivity. Therefore, the decrease of GDla in COS7 cell membrane upon MmNEU3 over-expression is likely to be instrumental to NDV reduced infection. Evidence was also provided for the preferential association of NDV-HN at 4 degrees C to detergent resistant microdomains (DRMs) of COS7 cells plasma membranes. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:504 / 512
页数:9
相关论文
共 32 条
[11]  
Lamb R.A., 2001, Fields virology, P1305
[12]   Identification of gangliosides GD1b and GT1b as receptors for BK virus [J].
Low, JA ;
Magnuson, B ;
Tsai, B ;
Imperiale, MJ .
JOURNAL OF VIROLOGY, 2006, 80 (03) :1361-1366
[13]   Internalization of Echovirus 1 in caveolae [J].
Marjomäki, V ;
Pietiäinen, V ;
Matilainen, H ;
Upla, P ;
Ivaska, J ;
Nissinen, L ;
Reunanen, H ;
Huttunen, P ;
Hyypiä, T ;
Heino, J .
JOURNAL OF VIROLOGY, 2002, 76 (04) :1856-1865
[14]  
MARKWELL MAK, 1991, PARAMYXOVIRUSES, P407
[15]   The plasma membrane-associated sialidase MmNEU3 modifies the ganglioside pattern of adjacent cells supporting its involvement in cell-to-cell interactions [J].
Papini, N ;
Anastasia, L ;
Tringali, C ;
Croci, G ;
Bresciani, R ;
Yamaguchi, K ;
Miyagi, T ;
Preti, A ;
Prinetti, A ;
Prioni, S ;
Sonnino, S ;
Tettamanti, G ;
Venerando, B ;
Monti, E .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (17) :16989-16995
[16]   Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER [J].
Pelkmans, L ;
Kartenbeck, J ;
Helenius, A .
NATURE CELL BIOLOGY, 2001, 3 (05) :473-483
[17]   Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4+ T cells [J].
Popik, W ;
Alce, TM ;
Au, WC .
JOURNAL OF VIROLOGY, 2002, 76 (10) :4709-4722
[18]   Paramyxovirus receptor-binding molecules: Engagement of one site on the hemagglutinin-neuraminidase protein modulates activity at the second site [J].
Porotto, M ;
Fornabaio, M ;
Greengard, O ;
Murrell, MT ;
Kellogg, GE ;
Moscona, A .
JOURNAL OF VIROLOGY, 2006, 80 (03) :1204-1213
[19]   Modulation of entry of enveloped viruses by cholesterol and sphingolipids (Review) [J].
Rawat, SS ;
Viard, M ;
Gallo, SA ;
Rein, A ;
Blumenthal, R ;
Puri, A .
MOLECULAR MEMBRANE BIOLOGY, 2003, 20 (03) :243-254
[20]  
Riboni L, 2000, METHOD ENZYMOL, V311, P656