Synthesis of proteins by native chemical ligation using Fmoc-based chemistry

被引：37

作者：

Camarero, JA ^{[1
]}

Mitchell, AR ^{[1
]}

机构：

[1] Lawrence Livermore Natl Lab, Chem Biol & Nucl Sci Div, Livermore, CA 94550 USA

来源：

PROTEIN AND PEPTIDE LETTERS | 2005年 / 12卷 / 08期

关键词：

chemical protein synthesis; Native Chemical Ligation; peptide alpha-thioester; hydrazine linker; protein engineering; Fmoc-chemistry;

D O I：

10.2174/0929866054864166

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C-terminal peptide alpha-thioesters are valuable intermediates in the synthesis/semisynthesis of proteins by native chemical. ligation. They are prepared either by solid-phase peptide synthesis (SPPS) or biosynthetically by protein splicing techniques. The present paper reviews the different methods available for the chemical synthesis of peptide alpha-thioesters using Fmoc-based SPPS.

引用

页码：723 / 728

页数：6

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