Synthesis of proteins by native chemical ligation using Fmoc-based chemistry

被引:37
作者
Camarero, JA [1 ]
Mitchell, AR [1 ]
机构
[1] Lawrence Livermore Natl Lab, Chem Biol & Nucl Sci Div, Livermore, CA 94550 USA
关键词
chemical protein synthesis; Native Chemical Ligation; peptide alpha-thioester; hydrazine linker; protein engineering; Fmoc-chemistry;
D O I
10.2174/0929866054864166
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
C-terminal peptide alpha-thioesters are valuable intermediates in the synthesis/semisynthesis of proteins by native chemical. ligation. They are prepared either by solid-phase peptide synthesis (SPPS) or biosynthetically by protein splicing techniques. The present paper reviews the different methods available for the chemical synthesis of peptide alpha-thioesters using Fmoc-based SPPS.
引用
收藏
页码:723 / 728
页数:6
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