Histone H1 is released from myonuclei and present in rimmed vacuoles with DNA in inclusion body myositis

被引：19

作者：

Nakano, Satoshi ^{[1
]}

Shinde, Akiyo ^{[1
]}

Fujita, Kengo ^{[1
]}

Ito, Hidefumi ^{[1
]}

Kusaka, Hirofumi ^{[1
]}

机构：

[1] Kansai Med Univ, Dept Neurol, Moriguchi, Osaka 5708507, Japan

来源：

NEUROMUSCULAR DISORDERS | 2008年 / 18卷 / 01期

基金：

日本学术振兴会;

关键词：

inclusion body myositis; distal myopathy; rimmed vacuole; nuclear degeneration; immunohistochemistry; pathology;

D O I：

10.1016/j.nmd.2007.08.005

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

To investigate myonuclear alterations in sporadic inclusion body myositis (s-IBM), we immuno-localized histones in muscles in I I patients. The examination showed that vacuolar rims were frequently positive for histone HI. In triple-color fluorescence study, the Hi-positive products were found on the inner side of an emerin-positive circle with DNA. Moreover, HI-positive materials appeared to be released into the cytoplasm in some vacuoles and myonuclei. The localization of HI was different from phosphorylated Elk-1, which is a nuclear protein, but abnormally accumulated in the cytoplasm in s-IBM. The results strongly support the hypothesis that rimmed vacuoles are derived from the nucleus. The cytoplasmic H I -release suggests dysfunction of nuclear membranes in an early phase of the nuclear disintegration. We hypothesize that, in s-IBM muscles, compromised nuclear envelope may permit release of some nuclear components such as histone HI and cannot facilitate the incorporation of others to the nucleus as in pElk-1. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：27 / 33

页数：7

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