Structural and functional analysis of pTB6 from Bifidobacterium longum

被引：33

作者：

Tanaka, K ^{[1
]}

Samura, K ^{[1
]}

Kano, Y ^{[1
]}

机构：

[1] Kyoto Pharmaceut Univ, Inst Mol & Cellular Biol Pharmaceut Sci, Dept Mol Genet, Yamashima Ku, Kyoto 6078412, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2005年 / 69卷 / 02期

关键词：

Bifidobacterium longum; nucleovide sequence of pTB6; RepB and oriV; immobile plasmid; high-efficient transformation;

D O I：

10.1271/bbb.69.422

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The complete nucleotide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB aa sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44, suggesting that pTB6 replicates by the rolling circle mechanism. The putative MembB, MobA, and protein encoding from orf (Orf) I detected were nonessential for plasmid replication. We constructed an immobile shuttle vector from pTB6 and pUC18, which transformed B. longum with a high efficiency of 2.5 X 10(6) transformants/mug DNA.

引用

收藏

页码：422 / 425

页数：4

相关论文

共 14 条

[1]

Molecular characterization of three plasmids from Bifidobacterium longum [J].

Corneau, N ;

Émond, É ;

LaPointe, G .

PLASMID, 2004, 51 (02) :87-100

[2]

EFFECTIVENESS OF BIFIDOBACTERIUM-BIFIDUM IN MEDIATING THE CLINICAL COURSE OF MURINE ROTAVIRUS DIARRHEAL [J].

DUFFY, LC ;

ZIELEZNY, MA ;

RIEPENHOFFTALTY, M ;

DRYJA, D ;

SAYAHTAHERIALTAIE, S ;

GRIFFITHS, E ;

RUFFIN, D ;

BARRETT, H ;

ROSSMAN, J ;

OGRA, PL .

PEDIATRIC RESEARCH, 1994, 35 (06) :690-695

[3]

A classification scheme for mobilization regions of bacterial plasmids [J].

Francia, MV ;

Varsaki, A ;

Garcillán-Barcia, MP ;

Latorre, A ;

Drainas, C ;

de la Cruz, F .

FEMS MICROBIOLOGY REVIEWS, 2004, 28 (01) :79-100

[4]

Fujimori M, 2002, CURR OPIN DRUG DI DE, V5, P200

[5]

INHIBITION OF ESCHERICHIA-COLI BY BIFIDOBACTERIA [J].

IBRAHIM, SA ;

BEZKOROVAINY, A .

JOURNAL OF FOOD PROTECTION, 1993, 56 (08) :713-715

[6]

Construction of Escherichia coli Bifidobacterium longum shuttle vector transforming B-longum 105-A and 108-A [J].

Matsumura, H ;

Takeuchi, A ;

Kano, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1997, 61 (07) :1211-1212

[7]

Mitsuoka E. T., 1992, LACTIC ACID BACTERIA, V1, P69, DOI DOI 10.1007/978-1-4615-3522-5

[8]

Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors [J].

Nakamura, T ;

Sasaki, T ;

Fujimori, M ;

Yazawa, K ;

Kano, Y ;

Amano, J ;

Taniguchi, S .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2002, 66 (11) :2362-2366

[9]

REDDY BS, 1993, CANCER RES, V53, P3914

[10]

FEEDING OF BIFIDOBACTERIUM-BIFIDUM AND STREPTOCOCCUS-THERMOPHILUS TO INFANTS IN-HOSPITAL FOR PREVENTION OF DIARRHEA AND SHEDDING OF ROTAVIRUS [J].

SAAVEDRA, JM ;

BAUMAN, NA ;

OUNG, I ;

PERMAN, JA ;

YOLKEN, RH .

LANCET, 1994, 344 (8929) :1046-1049