A prototypic microfluidic platform generating stepwise concentration gradients for real-time study of cell apoptosis

被引：29

作者：

Dai, Wen ^{[1
,2
]}

Zheng, Yizhe ^{[1
]}

Luo, Kathy Qian ^{[3
]}

Wu, Hongkai ^{[1
,2
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Chem, Kowloon, Hong Kong, Peoples R China

[2] Hong Kong Univ Sci & Technol, NSNT Program, Kowloon, Hong Kong, Peoples R China

[3] Nanyang Technol Univ, Sch Chem & Biomed Engn, Div Bioengn, Singapore 637457, Singapore

来源：

BIOMICROFLUIDICS | 2010年 / 4卷 / 02期

关键词：

HL60; CELLS; DEVICE; ACTIVATION; MICROCHIP; CHEMOTAXIS; INHIBITOR; VIABILITY; DYNAMICS; DOCKING; SYSTEMS;

D O I：

10.1063/1.3398319

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This work describes the development of a prototypic microfluidic platform for the generation of stepwise concentration gradients of drugs. A sensitive apoptotic analysis method is integrated into this microfluidic system for studying apoptosis of HeLa cells under the influence of anticancer drug, etoposide, with various concentrations in parallel; it measures the yellow fluorescent protein/cyan fluorescent protein fluorescence resonance energy transfer (FRET) signal that responds to the activation of caspase-3, an indicator of cell apoptosis. Sets of microfluidic valves on the chip generate stepwise concentration gradient of etoposide in various cell-culture microchambers. The FRET signals from multiple chambers are simultaneously monitored under a fluorescent microscope for long-time observation and the on-chip results are compared with those from 96-well plate study and the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The microfluidic platform shows several advantages including high-throughput capacity, low drug consumption, and high sensitivity. (C) 2010 American Institute of Physics. [doi:10.1063/1.3398319]

引用

页数：14