Detecting Key Genes Regulated by miRNAs in Dysfunctional Crosstalk Pathway of Myasthenia Gravis

被引：8

作者：

Cao, Yuze ^{[1
,2
]}

Wang, Jianjian ^{[1
]}

Zhang, Huixue ^{[1
]}

Tian, Qinghua ^{[1
]}

Chen, Lixia

Ning, Shangwei ^{[3
]}

Liu, Peifang ^{[1
]}

Sun, Xuesong ^{[1
]}

Lu, Xiaoyu ^{[1
]}

Song, Chang ^{[1
]}

Zhang, Shuai ^{[1
]}

Xiao, Bo ^{[2
]}

Wang, Lihua ^{[1
]}

机构：

[1] Harbin Med Univ, Affiliated Hosp 2, Dept Neurol, Harbin 150081, Heilongjiang, Peoples R China

[2] Cent South Univ, Xiangya Hosp, Dept Neurol, Changsha 410008, Hunan, Peoples R China

[3] Harbin Med Univ, Coll Bioinformat Sci & Technol, Harbin 150081, Heilongjiang, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2015年 / 2015卷

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金; 美国国家科学基金会;

关键词：

MICRORNA TARGET PREDICTION; LONG-TERM POTENTIATION; MAMMALIAN MICRORNAS; EXPRESSION; CELLS; IDENTIFICATION; ACTIVATION; DATABASE; DIFFERENTIATION; AUTOANTIBODIES;

D O I：

10.1155/2015/724715

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Myasthenia gravis (MG) is a neuromuscular autoimmune disorder resulting from autoantibodies attacking components of the neuromuscular junction. Recent studies have implicated the aberrant expression of microRNAs (miRNAs) in the pathogenesis of MG; however, the underlying mechanisms remain largely unknown. This study aimed to identify key genes regulated by miRNAs in MG. Six dysregulated pathways were identified through differentially expressed miRNAs and mRNAs in MG, and significant crosstalk was detected between five of these. Notably, crosstalk between the "synaptic long-term potentiation" pathway and four others was mediated by five genes involved in the MAPK signaling pathway. Furthermore, 14 key genes regulated by miRNAs were detected, of which six-MAPK1, RAF1, PGF, PDGFRA, EP300, and PPP1CC-mediated interactions between the dysregulated pathways. MAPK1 and RAF1 were responsible for most of this crosstalk (80%), likely reflecting their central roles in MG pathogenesis. In addition, most key genes were enriched in immune-related local areas that were strongly disordered in MG. These results provide new insight into the pathogenesis of MG and offer new potential targets for therapeutic intervention.

引用

页数：10

共 70 条

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