Strategy for anti-aquaporin-4 auto-antibody identification and quantification using a new cell-based assay

被引：25

作者：

De Vidi, I. ^{[1
,2
]}

Boursier, G. ^{[1
]}

Delouche, N. ^{[1
]}

Portales, P. ^{[1
]}

Cadars, E. ^{[1
]}

Bouthier, M. ^{[1
]}

Mettling, C. ^{[2
]}

Lin, Y. L. ^{[2
]}

Thouvenot, E. ^{[3
]}

Carlander, B. ^{[3
]}

Camu, W. ^{[3
]}

Antel, J. P. ^{[4
]}

Bar-Or, A. ^{[4
]}

Zephir, H. ^{[5
]}

Vermersch, P. ^{[5
]}

De Seze, J. ^{[6
]}

Corbeau, P. ^{[2
]}

Eliaou, J. F. ^{[1
]}

Vincent, T. ^{[1
,2
]}

机构：

[1] CHU Montpellier, Hop St Eloi, Dept Immunol, F-34295 Montpellier 5, France

[2] CNRS, Inst Genet Humaine, UPR1142, Montpellier, France

[3] CHU Montpellier, Hop Gui de Chauliac, MS Clin, Serv Neurol, F-34295 Montpellier 5, France

[4] McGill Univ, Montreal Neurol Inst, Neuroimmunol Unit, Montreal, PQ, Canada

[5] Univ Lille Nord France, Lille, France

[6] CHU Strasbourg, Hop Civil, F-67000 Strasbourg, France

来源：

CLINICAL IMMUNOLOGY | 2011年 / 138卷 / 03期

关键词：

Neuromyelitis optica; NMO-IgG; Aquaporin-4; Auto-antibody; Cell-based assay; Flow cytometry; NEUROMYELITIS-OPTICA; MULTIPLE-SCLEROSIS; DIAGNOSTIC-CRITERIA; AQUAPORIN-4; ANTIBODIES; MARKER;

D O I：

10.1016/j.clim.2010.11.012

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

NMO-IgG is a specific biomarker of neuromyelitis optica (NMO) that targets the aquaporin-4 (AQP4) water channel protein. The current gold standard for NMO-IgG identification is indirect immunofluorescence (IF). Our aim in this study was to develop a new quantitative cell-based assay (CBA) and to propose a rational strategy for anti-AQP4 Ab identification and quantification. We observed an excellent correlation between the CBA and IIF for NMO-IgG/anti-AQP4 detection. The CBA appeared more sensitive than IIF but on the other hand, IIF allows the simultaneous detection of various auto-Abs, underlining the complementarity between both methods. In conclusion, we propose to use IIF for the screening of patients at diagnosis in order to identify auto-Abs targeting the central nervous system. A highly sensitive, AQP4 specific and quantitative assay such as our CBA could be used thereafter to specifically identify the target of the Ab and to monitor its serum concentration under treatment. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：239 / 246

页数：8

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