Role of poly(A) tail length in Alu retrotransposition

被引:64
作者
Dewannieux, M [1 ]
Heidmann, T [1 ]
机构
[1] Inst Gustave Roussy, CNRS, UMR 8122, Unite Retrovirus Endogenes & Elements Retroides E, F-94805 Villejuif, France
关键词
Alu; retrotransposition; pA tail; LINE; SINE;
D O I
10.1016/j.ygeno.2005.05.009
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Alu are mobile noncoding Short INterspersed Elements (SINEs) present at a million copies in the human genome. Using marked Alu sequences in an ex vivo assay, we previously showed that they are mobilized through diversion of the LNE (Long INterspersed Elements) retrotransposition machinery, with the poly(A) tail of the Alu being required for their mobility. Here we show that other homopolymeric tracts cannot functionally replace the Alu poly(A) tail, and that the Alu transposition rate varies over a two-log range depending on the poly(A) tail length. Variation is according to a sigmoid-shaped curve with a lag observed for tails shorter than 15 nt and a plateau reached for tails longer than 50 m, consistent with the binding of a limited number of a protein component requiring multiple contacts for a productive interaction with the poly(A) stretch. This analysis indicates that most of the naturally occurring genomic Alu, owing to their pA tail length, should be poor substrates for the LINE machinery, a feature possibly "selected" for the host sake. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:378 / 381
页数:4
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