siRNA as a route to new cancer therapies

被引：18

作者：

Ameyar-Zazoua, M ^{[1
]}

Guasconi, V ^{[1
]}

Ait-Si-Ali, S ^{[1
]}

机构：

[1] Andre Lwoff Inst, CNRS, UPR 9079, F-94800 Villejuif, France

来源：

EXPERT OPINION ON BIOLOGICAL THERAPY | 2005年 / 5卷 / 02期

关键词：

cancer; gene therapy; RNAi; siRNA;

D O I：

10.1517/14712598.5.2.221

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The RNA interference (RNAi) gene-silencing mechanism is induced by double-stranded RNA (dsRNA) and is highly sequence-specific. It is an extremely powerful tool for silencing gene expression in vitro, and might be used as therapy in human pathologies such as cancer, viral infections and genetic disorders. RNAi was initially discovered in plants, but it has become clear that it is also conserved in animal species. Triggering of RNAi by the introduction of small dsRNA (or small interfering RNA) into living cells as a tool to inhibit the expression of specific genes holds the promise to selectively extinguish the expression of disease-associated genes in humans. On the other hand, RNAi technology will serve to elucidate the functions and interactions of the thousands of human genes in high-throughput systems and help in target validation technology.

引用

页码：221 / 224

页数：4

共 14 条

[1] RNAi and related mechanisms and their potential use for therapy [J].

Agami, R .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2002, 6 (06) :829-834

[2] Blocking oncogenes in malignant cells by RNA interference - New hope for a highly specific cancer treatment? [J].

Borkhardt, A .

CANCER CELL, 2002, 2 (03) :167-168

[3] Stable suppression of tumorigenicity by virus-mediated RNA interference [J].

Brummelkamp, TR ;

Bernards, R ;

Agami, R .

CANCER CELL, 2002, 2 (03) :243-247

[4] Systematic genome-wide screens of gene function [J].

Carpenter, AE ;

Sabatini, DM .

NATURE REVIEWS GENETICS, 2004, 5 (01) :11-22

[5] Role of microRNAs in plant and animal development [J].

Carrington, JC ;

Ambros, V .

SCIENCE, 2003, 301 (5631) :336-338

[6] Specific inhibition of pathological prion protein accumulation by small interfering RNAs [J].

Daude, N ;

Marella, M ;

Chabry, J .

JOURNAL OF CELL SCIENCE, 2003, 116 (13) :2775-2779

[7] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

[8] Genetic requirements for inheritance of RNAi in C-elegans [J].

Grishok, A ;

Tabara, H ;

Mello, CC .

SCIENCE, 2000, 287 (5462) :2494-2497

[9] RNA interference [J].

Hannon, GJ .

NATURE, 2002, 418 (6894) :244-251

[10] Synthetic small inhibiting RNAs:: Efficient tools to inactivate oncogenic mutations and restore p53 pathways [J].

Martinez, LA ;

Naguibneva, I ;

Lehrmann, H ;

Vervisch, A ;

Tchénio, T ;

Lozano, G ;

Harel-Bellan, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14849-14854

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