Direct chiral resolution of tartaric acid in food products by ligand exchange capillary electrophoresis using copper(Il)-D-quinic acid as a chiral selector

被引：49

作者：

Kodama, S

Yamamoto, A

Matsunaga, A

Hayakawa, K

机构：

[1] Toyama Inst Hlth, Toyama 9390363, Japan

[2] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 932卷 / 1-2期

关键词：

enantiomer separation; food analysis; ligand-exchange capillary electrophoresis; tartaric acid; quinic acid;

D O I：

10.1016/S0021-9673(01)01228-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chiral resolution of native DL-tartaric acid was performed by ligand-exchange capillary electrophoresis using copper(II)-D-quinic acid as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of tartaric acid were studied. The running conditions for optimum separation of tartaric acid were found to be 1 mM copper(II) sulfate-10 MM D-quinic acid (pH 5.0) with an effective voltage of -15 kV at 30 degreesC, using direct detection at 250 nm, and resolution of racemic, tartaric acid was approximately 1.3. With this system, chiral resolution Of DL-tartaric acid in food products was conducted successfully. (C) 2001 Elsevier Science B.V. All rights reserved.

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页码：139 / 143

页数：5

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