A survey of quantitative real-time polymerase chain reaction internal reference genes for expression studies in Brassica napus

被引：103

作者：

Chen, Xue ^{[1
]}

Truksa, Martin ^{[1
]}

Shah, Saleh ^{[2
]}

Weselake, Randall J. ^{[1
]}

机构：

[1] Univ Alberta, Dept Agr Food & Nutr Sci, Agr Lipid Biotechnol Program, Edmonton, AB T6H 2P5, Canada

[2] Alberta Innovates Technol Futures, Plant Biotechnol, Vegreville, AB T9C 1T4, Canada

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 405卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

qRT-PCR; Reference gene; Brassica napus; Embryo; geNORM; SEED MATURATION; PCR; NORMALIZATION;

D O I：

10.1016/j.ab.2010.05.032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Eight reference genes of Brassica napus were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) data, focusing on vegetative tissues and developing embryos. Analyses of expression stability indicated that UP1, UBC9, UBC21, and TIP41 were the top four choices as stably expressed reference genes for vegetative tissues, whereas ACT7, UBC21, TIP41, and PP2A were the top four choices for maturing embryos. In addition, radiolabeling of overall messenger RNA (mRNA) of maturing embryos indicated that the expression patterns of the top four ranked reference genes reflected the overall mRNA content changes in maturing embryos. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.

引用

页码：138 / 140

页数：3

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