Involvement of the gapA- and epd (gapB)-encoded dehydrogenases in pyridoxal 5′-phosphate coenzyme biosynthesis in Escherichia coli K-12

被引：41

作者：

Yang, Y ^{[1
]}

Zhao, GS ^{[1
]}

Man, TK ^{[1
]}

Winkler, ME ^{[1
]}

机构：

[1] Univ Texas, Sch Med, Dept Microbiol & Mol Genet, Houston, TX 77030 USA

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 16期

关键词：

D O I：

10.1128/JB.180.16.4294-4299.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We show that epd (gapB) mutants lacking an erythrose 4-phosphate (E4P) dehydrogenase are impaired for growth on some media and contain less pyridoxal 5'-phosphate (PLP) and pyridoxamine 5'-phosphate (PMP) than their epd(+) parent. In contrast to a previous report, we found that gapA epd double mutants lacking the glyceraldehyde 3-phosphate and E4P dehydrogenases are auxotrophic for pyridoxine. These results implicate the GapA and Epd dehydrogenases in de novo PLP and PMP coenzyme biosynthesis.

引用

页码：4294 / 4299

页数：6

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