Advancing Crop Transformation in the Era of Genome Editing

被引：445

作者：

Altpeter, Fredy ^{[1
]}

Springer, Nathan M. ^{[2
]}

Bartley, Laura E. ^{[3
]}

Blechl, Ann E. ^{[4
]}

Brutnell, Thomas P. ^{[5
]}

Citovsky, Vitaly ^{[6
]}

Conrad, Liza J. ^{[7
]}

Gelvin, Stanton B. ^{[8
]}

Jackson, David P. ^{[9
]}

Kausch, Albert P. ^{[10
]}

Lemaux, Peggy G. ^{[11
]}

Medford, June I. ^{[12
]}

Orozco-Cardenas, Martha L. ^{[13
]}

Tricoli, David M. ^{[14
]}

Van Eck, Joyce ^{[15
]}

Voytas, Daniel F. ^{[16
,17
]}

Walbot, Virginia ^{[18
]}

Wang, Kan ^{[19
,20
]}

Zhang, Zhanyuan J. ^{[21
]}

Stewart, C. Neal, Jr. ^{[22
]}

机构：

[1] Univ Florida, IFAS, Dept Agron, Plant Mol & Cellular Biol Program, Gainesville, FL 32611 USA

[2] Univ Minnesota, Dept Plant Biol, Microbial & Plant Genom Inst, St Paul, MN 55108 USA

[3] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA

[4] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA

[5] Donald Danforth Plant Sci Ctr, Enterprise Inst Renewable Fuels, St Louis, MO 63132 USA

[6] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA

[7] Eckerd Coll, Nat Sci Collegium, St Petersburg, FL 33711 USA

[8] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

[9] Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA

[10] Univ Rhode Isl, Dept Cellular & Mol Biol, Kingston, RI 02881 USA

[11] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[12] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA

[13] Univ Calif Riverside, Plant Transformat Res Ctr, Riverside, CA 92521 USA

[14] Univ Calif Davis, Plant Transformat Facil, Davis, CA 95616 USA

[15] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA

[16] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

[17] Univ Minnesota, Ctr Genome Engn, Minneapolis, MN 55455 USA

[18] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[19] Iowa State Univ, Inst Plant Sci, Dept Agron, Ames, IA 50011 USA

[20] Iowa State Univ, Inst Plant Sci, Ctr Plant Transformat, Ames, IA 50011 USA

[21] Univ Missouri, Div Plant Sci, Plant Transformat Core Facil, Columbia, MO 65211 USA

[22] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA

来源：

PLANT CELL | 2016年 / 28卷 / 07期

基金：

美国国家科学基金会;

关键词：

AGROBACTERIUM-MEDIATED TRANSFORMATION; PLANT SYNTHETIC BIOLOGY; GENETIC-TRANSFORMATION; T-DNA; TRANSCRIPTION FACTOR; VIRD2; PROTEIN; SOMATIC EMBRYOGENESIS; PARTICLE BOMBARDMENT; TRANSGENIC PLANTS; TUMEFACIENS VIRD2;

D O I：

10.1105/tpc.16.00196

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than 30 years of technological advances. Genome editing provides novel opportunities to enhance crop productivity but relies on genetic transformation and plant regeneration, which are bottlenecks in the process. Here, we review the state of plant transformation and point to innovations needed to enable genome editing in crops. Plant tissue culture methods need optimization and simplification for efficiency and minimization of time in culture. Currently, specialized facilities exist for crop transformation. Single-cell and robotic techniques should be developed for high-throughput genomic screens. Plant genes involved in developmental reprogramming, wound response, and/or homologous recombination should be used to boost the recovery of transformed plants. Engineering universal Agrobacterium tumefaciens strains and recruiting other microbes, such as Ensifer or Rhizobium, could facilitate delivery of DNA and proteins into plant cells. Synthetic biology should be employed for de novo design of transformation systems. Genome editing is a potential game-changer in crop genetics when plant transformation systems are optimized.

引用

页码：1510 / 1520

页数：11

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